Mário Cunha

Abstract
Processes shaping urban ecosystems reflect and influence the cultural context in which they emerge, bearing implications for ecosystem services (ES) planning and management. Investigating the perception of benefits and losses / costs delivered by a specific service providing unit (SPU) can generate objective orientations suitable for urban planning and management deeply embedded in the social-ecological systems where they occur, because the realization of ES into benefits and losses / costs is mediated by specific beneficiaries and reflects their characteristics, information and use of ecosystems. Street trees are a particularly relevant SPU in many densely built Southern-European cities due to the difficulty in implementing new sizeable green areas. In this study, a questionnaire was developed and applied in Porto to investigate how benefits (cultural, regulating and economic) and losses / costs caused by street trees are perceived by citizens and influenced by a set of socioeconomic variables (N = 819 people aged 18 years or older), and parametric statistical tests were used to analyze the effect of gender, age and school level. Results evidenced that people in Porto valued more environmental benefits (particularly air quality improvement) than cultural ones. School level was the variable accounting for more differences, underlining a tendency in people with lower level of academic education to value less the benefits provided by street trees in Porto and attribute more importance to losses and damages, compared to people who attended university or had higher academic degree. Age also held considerable differences in mean responses, with older people showing more concern towards losses and costs, while gender influenced perception of cultural benefits, which were more important for women than for men. The findings of the research are discussed concerning implications for environmental justice, planning and management of urban ecosystems. © 2018 Elsevier GmbH

Abstract
Significant advances have been made in identifying, quantifying and valuing multiple urban ecosystem services (UES), yet this knowledge remains poorly implemented in urban planning and management. One of the reasons for this low implementation is the insufficient thematic and spatial detail in UES research to provide guidance for urban planners and managers. Acknowledging how patterns of UES delivery are related with vegetation structure and composition in urban green areas could help these stakeholders to target structural variables that increase UES provision. This investigation explored how different types of urban green spaces influence UES delivery in Porto, a Portuguese city, and how this variation is affected by a socioeconomic gradient. A stepwise approach was developed using two stratification schemes and a modelling tool to estimate urban forest structure and UES provision. This approach mapped explicit cold and hotspots of UES provision and discriminated the urban forest structural variables that influence UES at the local scale. Results revealed that different types of green spaces affect UES delivery as a direct result of the influence of structural variables of the urban forest. Furthermore, the uneven distribution of green spaces types across socioeconomic strata alters UES delivery across the city. This case study illustrates how a methodology adaptable to other geographic contexts can be used to map and analyze coupled social and ecological patterns, offering novel insights that are simple to understand and apply by urban planners and managers.

Abstract
Pollen quality of 15 cultivars of Vitis vinifera L. was studied in this work. Pollen viability was tested by the fluorochromatic reaction and germination was analyzed by in vitro assays, using two different media. Differences among cultivars in the number of pollen apertures were observed under light microscope. All the cultivars studied showed a higher percentage of tricolporated pollen, however, pollen grains containing one, two or four apertures were also observed. The cultivar Loureiro was the one with the higher percentage of pollen grains with four apertures (3.8%) and Touriga Nacional presented 100% of tricolporated pollen grains. The viability analysis showed that 13 cultivars presented values higher than 50%, with 8 cultivars reaching values above 75%. The pollen germination rates vary greatly for the grapevine cultivars studied, three cultivars show low values of germination (under 14%) in the two media tested, which were Touriga Nacional, Cabernet Franc, and Cabernet Sauvignon while others presented high values of germination like Casteldo, Loureiro, Malbec and Petit Verdot. No significant statistical differences between the percentages of germination in the two media studied were found for the majority of cultivars analyzed.

Abstract
In this work, fungi present in the grapevine's phyllosphere collected from the main demarcated wine regions of Portugal were identified, and their phylogenetic relationships were analysed. A total of 46 vine samples (leaves and berries) were collected from different parts of the country, being isolated a total of 117 fungal colonies that were identified to the genus level and sequenced in the following genetic regions: internal transcribed spacer region and 18S rRNA and -tubulin gene. Next, a phylogenetic tree reconstruction for each genetic region was built. The isolates retrieved from environmental samples belonged to the genera Alternaria (31%), Cladosporium (21%), Penicillium (19%), Aspergillus (7%) and Epicoccum (3%). No genetic signatures of exchange of genetic material were detected, and consequently, the reconstructed phylogenetic trees allowed to distinguish between these different species/genera. In the fungal composition of the Vitis vinifera phyllosphere, several potential pathogens were identified that can be associated with decreases in crop productivity. Knowledge of fungi identification and genetic diversity is pivotal for the development of more adequate crop management strategies. Furthermore, this information will provide guidelines for a more specific and wiser use of fungicides. Significance and Impact of the StudyThe knowledge on the composition of the phyllosphere microbial community is still limited, especially when fungi are concerned. These micro-organisms not only play a crucial role in crop health and productivity but also interact with the winemaking process, determining the safety and quality of grape and grape-derived products. The elucidation of the micro-organisms present in the phyllosphere will have a notorious impact on plant breeding and protection programmes and disease management strategies, allowing a better control of pesticide applications.

Abstract