Mário Cunha

Abstract
The spatial monitoring of plant diversity in the endangered species-rich grasslands of European mountain pastoral systems is an important step for fairer and more efficient Agri-Environmental policy schemes supporting conservation. This study assessed the underlying support for a spatially explicit monitoring of plant species richness at parcel level (policy making scale) in Southern European mountain grasslands, with statistical models informed by Sentinel-2 satellite and environmental factors. Twenty-four grassland parcels were surveyed for species richness in the Peneda-Gerês National Park, northern Portugal. Using a multi-model inference approach, three competing hypotheses guided by the species-scaling theoretical framework were established: species–area (P1), species–energy (P2) and species–spectral heterogeneity (P3), each representing a candidate spatial pathway to predict species richness. To evaluate the statistical support of each spatial pathway, generalized linear models were fitted and model selection based on Akaike information criterion (AIC) was conducted. Later, the performance of the most supported spatial pathway(s) was assessed using a leave-one-out cross validation. A model guided by the species–energy hypothesis (P2) was the most parsimonious spatial pathway to monitor plant species richness in mountain grassland parcels (P2, AICc = 137.6, ?AIC = 0.0, wi = 0.97). Species–area and species–spectral heterogeneity pathways (P1 and P3) were less statistically supported (?AICc values in the range 5.7–10.0). The underlying support of the species–energy spatial pathway was based on Sentinel-2 satellite data, namely on the near-infrared (NIR) green ratio in the spring season (NIR/Greenspring) and on its ratio of change between spring and summer (NIR/Greenchange). Both predictor variables related negatively to species richness. Grassland parcels with lower values of near-infrared (NIR) green ratio and lower seasonal amplitude presented higher species richness records. The leave-one-out cross validation indicated a moderate performance of the species–energy spatial pathway in predicting species richness in the grassland parcels covered by the dataset (R2 = 0.44, RMSE = 4.3 species, MAE = 3.5 species). Overall, a species–energy framework based on Sentinel 2 data resulted in a promising spatial pathway for the monitoring of species richness in mountain grassland parcels and for informing decision making on Agri-Environmental policy schemes. The near-infrared (NIR) green ratio and its change in time seems a relevant variable to deliver predictions for plant species richness and further research should be conducted on that.

Abstract

Abstract
The harvesting operation is a recurring task in the production of any crop, thus making it an excellent candidate for automation. In protected horticulture, one of the crops with high added value is tomatoes. However, its robotic harvesting is still far from maturity. That said, the development of an accurate fruit detection system is a crucial step towards achieving fully automated robotic harvesting. Deep Learning (DL) and detection frameworks like Single Shot MultiBox Detector (SSD) or You Only Look Once (YOLO) are more robust and accurate alternatives with better response to highly complex scenarios. The use of DL can be easily used to detect tomatoes, but when their classification is intended, the task becomes harsh, demanding a huge amount of data. Therefore, this paper proposes the use of DL models (SSD MobileNet v2 and YOLOv4) to efficiently detect the tomatoes and compare those systems with a proposed histogram-based HSV colour space model to classify each tomato and determine its ripening stage, through two image datasets acquired. Regarding detection, both models obtained promising results, with the YOLOv4 model standing out with an F1-Score of 85.81%. For classification task the YOLOv4 was again the best model with an Macro F1-Score of 74.16%. The HSV colour space model outperformed the SSD MobileNet v2 model, obtaining results similar to the YOLOv4 model, with a Balanced Accuracy of 68.10%.

Abstract
Precisely monitoring the growth condition and nutritional status of maize is crucial for optimizing agronomic management and improving agricultural production. Multi-spectral sensors are widely applied in ecological and agricultural domains. However, the images collected under varying weather conditions on multiple days show a lack of data consistency. In this study, the Mini MCA 6 Camera from UAV platform was used to collect images covering different growth stages of maize. The empirical line calibration method was applied to establish generic equations for radiometric calibration. The coefficient of determination (R-2) of the reflectance from calibrated images and ASD Handheld-2 ranged from 0.964 to 0.988 (calibration), and from 0.874 to 0.927 (validation), respectively. Similarly, the root mean square errors (RMSE) were 0.110, 0.089, and 0.102% for validation using data of 5 August, 21 September, and both days in 2019, respectively. The soil and plant analyzer development (SPAD) values were measured and applied to build the linear regression relationships with spectral and textural indices of different growth stages. The Stepwise regression model (SRM) was applied to identify the optimal combination of spectral and textural indices for estimating SPAD values. The support vector machine (SVM) and random forest (RF) models were independently applied for estimating SPAD values based on the optimal combinations. SVM performed better than RF in estimating SPAD values with R-2 (0.81) and RMSE (0.14), respectively. This study contributed to the retrieval of SPAD values based on both spectral and textural indices extracted from multi-spectral images using machine learning methods.

Abstract
The interest in expanding the production of hops outside the traditional cultivation regions, mainly motivated by the growth of the craft brewery business, justifies the intensification of studies into its adaptation to local growing conditions. In this study, four field trials were undertaken on a twenty-year-old hop garden, over periods of up to three years to assess the effect of important agro-environmental variation factors on hop phenol and phenolic composition and to establish its relationship with the elemental composition of hop cones. All the field trials were arranged as factorial designs exploring the combined effect of: (1) plots of different vigour plants × year; (2) plots of different plant vigor × algae- and nutrient-rich foliar sprays × year; (3) plot × liming × year; and (4) cultivars (Nugget, Cascade, Columbus) × year. Total phenols in hops, were significantly influenced by most of the experimental factors. Foliar spraying and liming were the factors that least influenced the measured variables. The year had the greatest effect on the accumulation of total phenols in hop cones in the different trials and may have contributed to interactions that often occurred between the factors under study. The year average for total phenol concentrations in hop cones ranged from 11.9 mg g-1 to 21.2 mg g-1. Significant differences in quantity and composition of phenolic compounds in hop cones were also found between cultivars. The phenolic compounds identified were mainly flavonols (quercetin and kaempferol glycosides) and phenolic carboxylic acids (p-coumaric and caffeic acids).