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Publications

2014

DNA-Aptamer optical biosensors based on a LPG-SPR optical fiber platform for point-of care diagnostic

Authors
Coelho, L; Queiros, RB; Santos, JL; Martins, MCL; Viegas, D; Jorge, PAS;

Publication
PLASMONICS IN BIOLOGY AND MEDICINE XI

Abstract
Surface Plasmon Resonance (SPR) is the base for some of the most sensitive label free optical fiber biosensors. However, most solutions presented to date require the use of fragile fiber optic structure such as adiabatic tapers or side polished fibers. On the other hand, long-period fiber gratings (LPG) present themselves as an interesting solution to attain an evanescent wave refractive index sensor platform while preserving the optical fiber integrity. The combination of these two approaches constitute a powerful platform that can potentially reach the highest sensitivities as it was recently demonstrated by detailed theoretical study [1, 2]. In this work, a LPG-SPR platform is explored in different configurations (metal coating between two LPG - symmetric and asymmetric) operating in the telecom band (around 1550 nm). For this purpose LPGs with period of 396 mu m are combined with tailor made metallic thin films. In particular, the sensing regions were coated with 2 nm of chromium to improve the adhesion to the fiber and 16 nm of gold followed by a 100 nm thick layer of TiO2 dielectric material strategically chosen to attain plasmon resonance in the desired wavelength range. The obtained refractometric platforms were then validated as a biosensor. For this purpose the detection of thrombin using an aptamer based probe was used as a model system for protein detection. The surface of the sensing fibers were cleaned with isopropanol and dried with N-2 and then the aminated thrombin aptamer (5'-[NH2]-GGTTGGTGTGGTTGG-3') was immobilized by physisorption using Poly-L-Lysine (PLL) as cationic polymer. Preliminary results indicate the viability of the LPFG-SPR-APTAMER as a flexible platforms point of care diagnostic biosensors.

2014

Gas sensing using wavelength modulation spectroscopy

Authors
Viveiros, D; Ribeiro, J; Flores, D; Ferreira, J; Frazao, O; Santos, JL; Baptista, JM;

Publication
SECOND INTERNATIONAL CONFERENCE ON APPLICATIONS OF OPTICS AND PHOTONICS

Abstract
An experimental setup has been developed for different gas species sensing based on the Wavelength Modulation Spectroscopy (WMS) principle. The target is the measurement of ammonia, carbon dioxide and methane concentrations. The WMS is a rather sensitive technique for detecting atomic/molecular species presenting the advantage that it can be used in the near-infrared region using optical telecommunications technology. In this technique, the laser wavelength and intensity are modulated applying a sine wave signal through the injection current, which allows the shift of the detection bandwidth to higher frequencies where laser intensity noise is reduced. The wavelength modulated laser light is tuned to the absorption line of the target gas and the absorption information can be retrieved by means of synchronous detection using a lock-in amplifier, where the amplitude of the second harmonic of the laser modulation frequency is proportional to the gas concentration. The amplitude of the second harmonic is normalised by the average laser intensity and detector gain through a LabVIEW (R) application, where the main advantage of normalising is that the effects of laser output power fluctuations and any variations in laser transmission, or optical-electrical detector gain are eliminated. Two types of sensing heads based on free space light propagation with different optical path length were used, permitting redundancy operation and technology validation.

2014

On Expressing Strategies for Directive-Driven Multicore Programing Models

Authors
Nobre, R; Pinto, P; Carvalho, T; Cardoso, JMP; Diniz, PC;

Publication
PARMA-DITAM@HiPEAC

Abstract
A common migration path for applications to high-performance multicore architectures relies on code annotations with concurrent semantics. Some annotations, however, are very target architecture specific and thus highly non-portable. In this paper we describe a source-to-source code transformation system that allows programmers to specify transformations using an aspect-oriented domain specific language - LARA. LARA allows programmers to specify strategies to search large code transformation design spaces while preserving the original source code. As the experimental results reveal, this approach leads to a substantial reduction in code maintenance costs, and promotes the portability of both programmers and performance. Copyright © 2014 ACM.

2014

All-Fiber Sensor Based on a Metallic Coated Hybrid LPG-FBG Structure for Thermal Characterization of Materials

Authors
Silva, GE; Caldas, P; Santos, JC; Santos, JL;

Publication
23RD INTERNATIONAL CONFERENCE ON OPTICAL FIBRE SENSORS

Abstract
In this paper it is presented an all-fiber implementation of the hot-wire needle probe concept, widely used to measure the thermal properties of materials, particularly the thermal conductivity. It is based on the heating of a metal thin film deposited on the surface of the fiber induced by the coupling of laser light into the cladding via a long period grating, and determination, using a fiber Bragg grating, of the time dependence of the temperature of the surrounding medium at a fixed distance of the fiber. The medium considered in this research was the air and the results obtained indicate the feasibility of this approach and point out future developments.

2014

Vision-based gesture recognition system for human-computer interaction

Authors
Trigueiros, P; Ribeiro, F; Reis, LP;

Publication
COMPUTATIONAL VISION AND MEDICAL IMAGE PROCESSING IV

Abstract
Hand gesture recognition, being a natural way of human computer interaction, is an area of active research in computer vision and machine learning. This is an area with many different possible applications, giving users a simpler and more natural way to communicate with robots/systems interfaces, without the need for extra devices. So, the primary goal of gesture recognition research is to create systems, which can identify specific human gestures and use them to convey information or for device control. This work intends to study and implement a solution, generic enough, able to interpret user commands, composed of a set of dynamic and static gestures, and use those solutions to build an application able to work in a real-time human-computer interaction systems. The proposed solution is composed of two modules controlled by a FSM (Finite State Machine): a real time hand tracking and feature extraction system, supported by a SVM (Support Vector Machine) model for static hand posture classification and a set of HMMs (Hidden Markov Models) for dynamic single stroke hand gesture recognition. The experimental results showed that the system works very reliably, being able to recognize the set of defined commands in real-time. The SVM model for hand posture classification, trained with the selected hand features, achieved an accuracy of 99,2%. The proposed solution as the advantage of being computationally simple to train and use, and at the same time generic enough, allowing its application in any robot/system command interface.

2014

The project risk management process, a preliminary study

Authors
Rodrigues da Silva, LH; Crispim, JA;

Publication
CENTERIS 2014 - CONFERENCE ON ENTERPRISE INFORMATION SYSTEMS / PROJMAN 2014 - INTERNATIONAL CONFERENCE ON PROJECT MANAGEMENT / HCIST 2014 - INTERNATIONAL CONFERENCE ON HEALTH AND SOCIAL CARE INFORMATION SYSTEMS AND TECHNOLOGIES

Abstract
A process of risk management in projects is a rational chain of practices by which decision-agents plan and execute actions and control the results in order to keep the implementation of the project under certain conditions (time, cost and quality parameters' set). With the purpose of providing guidelines for the selection of the best practices taking into account the organizational maturity and project complexity, a theoretical framework to classify and associate those practices to each phase of the project life cycle and to each project risk management process is proposed. Future research efforts will be directed towards refining the framework and testing it in multiple case studies. (C) 2014 The Authors. Published by Elsevier Ltd.

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