2023
Authors
Cunha, C; Assuncao, AS; Monteiro, CS; Leitao, C; Mendes, JP; Silva, S; Frazao, O; Novais, S;
Publication
2023 IEEE 7TH PORTUGUESE MEETING ON BIOENGINEERING, ENBENG
Abstract
Using surface resonance (SPR) as a sensitivity enhancer, this work describes the development of a transmissive multimode optical fiber sensor with a gold (Au) thin film that measures glucose concentration. The fiber's cladding was initially removed, and an Au layer was then sputtered onto its surface to simultaneously excite SPR and reflect light, making the SPR sensor extremely sensitive to changes in the environment's refractive index. A range of glucose concentrations, from 0.0001 to 0.5000 g/ml, were tested on the sensor. A maximum sensitivity of 161.302 nm/(g/mL) was attained for the lowest glucose concentration, while the highest concentration yielded a sensitivity of 312.000 nm/(g/mL). The proposed sensor's compact size, high sensitivity, good stability and practicality make it a promising candidate for a range of applications, including detecting diabetes.
2023
Authors
Dos Santos, PSS; Mendes, JP; Dias, B; Perez-Juste, J; De Almeida, JMMM; Pastoriza-Santos, I; Coelho, LCC;
Publication
SENSORS
Abstract
Biochemical-chemical sensing with plasmonic sensors is widely performed by tracking the responses of surface plasmonic resonance peaks to changes in the medium. Interestingly, consistent sensitivity and resolution improvements have been demonstrated for gold nanoparticles by analyzing other spectral features, such as spectral inflection points or peak curvatures. Nevertheless, such studies were only conducted on planar platforms and were restricted to gold nanoparticles. In this work, such methodologies are explored and expanded to plasmonic optical fibers. Thus, we study-experimentally and theoretically-the optical responses of optical fiber-doped gold or silver nanospheres and optical fibers coated with continuous gold or silver thin films. Both experimental and numerical results are analyzed with differentiation methods, using total variation regularization to effectively minimize noise amplification propagation. Consistent resolution improvements of up to 2.2x for both types of plasmonic fibers are found, demonstrating that deploying such analysis with any plasmonic optical fiber sensors can lead to sensing resolution improvements.
2023
Authors
Vasconcelos, H; Matias, A; Mendes, J; Araujo, J; Dias, B; Jorge, PAS; Saraiva, C; de Almeida, JMMM; Coelho, LCC;
Publication
TALANTA
Abstract
Hydrogen peroxide is usually added to products to delay the development of microorganisms mainly in milk, hence increasing its stability over time, however the side effects can become devastating to human health.A technique is presented consisting of detecting hydrogen peroxide as an adulterant in milk through a sensor where pretreatment of the sample is not necessary, using a single use membrane. The detection of hydrogen peroxide in fresh-raw, whole, semi-skimmed and skimmed milk was performed using a luminol chem-iluminescence reaction.For hydrogen peroxide water solutions, a linear response was attained from 1.0 x 10-4 to 9.0 x 10-3 %w/w and an LOD (limit of detection) of 3.0 x 10-5 %w/w was determined. An R-squared value of 0.97 and a relative standard deviation lower than 10%, were achieved.Hydrogen peroxide concentration as low as 1.0 x 10-3 %w/w was measured for fresh-raw, skim and whole milk and for semi-skimmed milk, as low as 2.0 x 10-3 %w/w.The methodology presented, as long as our knowledge, is original, rapid, ecological and inexpensive. In regard of the sensitivity obtained, the methodology has great possibility to be applied in the detection of hydrogen peroxide in several areas. It is envisaged monitoring of food quality, agriculture systems and environment pollution.
2026
Authors
Pitruzzella, R; Cicatiello, D; Marzano, C; Passeggio, F; Gentile, L; Ribeiro, JA; Mendes, JP; Coelho, LCC; Portella, G; Capellupo, MC; Casale, M; Zeni, L; Jorge, PAS; Cennamo, N;
Publication
NANOMATERIALS
Abstract
Haemoglobin (Hb) concentration is a key biomarker for several diseases. Traditional laboratory methods often have limitations due to their time-consuming nature, the need for skilled personnel, or the use of high-cost instrumentation. This work presents a sensing strategy for developing new point-of-care tests (POCTs) for Hb detection via a proof of concept. The proposed sensing approach is implemented using plasmonic plastic optical fiber (POF) sensor chips that integrate an electropolymerized molecularly imprinted polymer (eMIP) film on the plasmonic surface for Hb-selective detection. The developed sensor system demonstrates an ultra-low detection limit of 80 fM in buffer, about five orders of magnitude lower than that of other comparable Hb sensors. Selectivity tests against common interfering proteins, such as bovine serum albumin (BSA) and immunoglobulin G (IgG), confirmed high specificity towards the target analyte. Moreover, the sensor's performance was tested using a whole-blood sample, yielding results consistent with those of standard haematology analysis. The proposed sensor system, based on simple equipment, provides a quick (about 10 min) and cost-effective (about 10 euros per chip) label-free diagnostic tool for POCTs in real-world scenarios, such as finger-prick sampling, offering a less invasive alternative to traditional laboratory methods, towards devices useful for Internet of Medical Things (IoMT).
2026
Authors
Santos, AD; Almeida, MAS; Mendes, JP; Almeida, JMMMd; Coelho, LCC;
Publication
Sensors
Abstract
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