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We implemented Discord as a pedagogical tool in the academic year of 2021/2022 in two mathematics curricular units of the first year of an Informatics Engineering university program. We analyze and discuss the experience, reflecting on usability and influence on learning processes and engagement. We compare the impact of using the platform: 1) when combined with different methodological and pedagogical approaches; and 2) to previous years with other (or none) classic virtual forums.

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Enhancing the reproducibility and comprehension of adaptive immune receptor repertoire sequencing (AIRR-seq) data analysis is critical for scientific progress. This study presents guidelines for reproducible AIRR-seq data analysis, and a collection of ready-to-use pipelines with comprehensive documentation. To this end, ten common pipelines were implemented using ViaFoundry, a user-friendly interface for pipeline management and automation. This is accompanied by versioned containers, documentation and archiving capabilities. The automation of pre-processing analysis steps and the ability to modify pipeline parameters according to specific research needs are emphasized. AIRR-seq data analysis is highly sensitive to varying parameters and setups; using the guidelines presented here, the ability to reproduce previously published results is demonstrated. This work promotes transparency, reproducibility, and collaboration in AIRR-seq data analysis, serving as a model for handling and documenting bioinformatics pipelines in other research domains.

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