Abstract
Among the groups of naturally occurring pigments, carotenoids serve numerous purposes in plants, including antioxidant activity and protection of the photosynthetic apparatus from the excess of light. Alongside with essential mineral elements, these pigments are well known to have high impact on human health. Therefore, accumulation of carotenoids in wheat grain is an important trait in view to improving nutritional value of cereals. Wheat is one of the major staple foods in Portugal because of its agronomical adaptability and the usage of its flour into various traditional products. In the present investigation, the seeds of ninety-four Old Portuguese wheat cultivars grown over two years were analysed. Significant genetic variability for content of carotenoids was observed in the studied lines. Within the 47 bread wheat genotypes the Yellow Pigment Content (YPC) values varied from 2.7 - 5.8 mu g/g and 3.0 - 8.0 mu g/g for 2004/2005 and 2009/2010, respectively. On the other hand, during 2004/2005 and 2009/2010, the 47 durum wheat cultivars exhibited the YPC values from 1.1 - 8.0 mu g/g and 3.4 - 8.3 mu g/g, respectively. In addition, multivariate methods were also explored to assess the wheat grains quality, resorting to FTIR spectroscopy (Figure 1). Preliminary analyses of FTIR spectra clearly revealed differences among the distinct studied genotypes. Thus, in order to find spectroscopical patterns related to carotenoids accumulation and antioxidant activity, efforts are being made to develop a model that will allow the assessment of these parameters through FTIR, in the near-future. (C) 2015 The Authors. Published by Elsevier B.V.

Abstract
Olive (Olea europaea L.) represents one of the most important and ancient crops in the Mediterranean countries, being widely known due to the optimal organoleptic characteristics of its oil, besides the important health benefits assigned to this product in the last few years. Since olive oil composition, respecting specific trace elements, has a direct impact on the quality and safety of this product, eleven monovarietal and twelve commercial olive oils were analysed spectrometrically concerning their metal composition. Regarding quality parameters, the monovarietal olive oils display larger quantities of Mg, Fe, Mn, while the commercial samples are characterized by greater amounts of Na, Ca, Cu and Zn. In which respects to safety parameters, the commercial samples present higher values for Al and Pb, and lower quantities of Cd, respecting the monovarietal ones. The assessment of the values observed for the trace elements, resorting to multivariate statistical methods, allowed to clearly distinguish the type of olive oil production monovarietal or commercial blend. For this purpose, besides Cluster analysis, the data have been subjected to Principal Component Analysis undertaken for either all the evaluated parameters simultaneously, or the quality/safety parameters separately, both data sets allowing the discrimination of the samples.

Abstract
The conformational preferences and hydrogen-bonding motifs of several potential chemopreventive hydroxycinnamic derivatives were determined by inelastic neutron scattering spectroscopy. The aim is to understand their recognized beneficial activity and establish reliable structure-activity relationships for these types of dietary phytochemicals. A series of phenolic acids with different hydroxyl/methoxyl ring substitution patterns were studied: trans-cinnamic, p-coumaric, m-coumaric, trans-caffeic and ferulic acids. Their INS spectra were completely assigned by theoretical calculations performed at the Density Functional Theory level, for the isolated molecule, dimeric centrosymmetric species and the solid (using plane-wave expansion approaches). Access to the low energy vibrational region of the spectra enabled the identification of particular modes associated with intermolecular hydrogen-bonding interactions, which are the determinants of the main conformational preferences and antioxidant capacity of these systems.

Abstract
In Mediterranean-like climate areas, field-grown grapevines are typically exposed to severe environmental conditions during the summer season, which can negatively impact the sustainability of viticulture. Despite the short-term mitigation strategies available nowadays to cope with climate change, little is known regarding their effectiveness in different demarcated winegrowing regions with differing climate features. Hence, we applied a kaolin suspension (5 %) to Touriga-Franca (TF) and Touriga-Nacional (TN) grapevine varieties located in two Portuguese demarcated regions (Alentejo and Douro) with different mesoclimates to study its effect on the physiological performance, hormonal balance and ABA-related grapevine leaf gene expression during the 2017 and 2018 growing seasons. Data show that 2017 was warmer than 2018 due to the occurrence of two heatwaves in both locations, highlighting the protective effect of kaolin application under severe environmental conditions. In the first study year, at midday, kaolin enhanced water use efficiency (23 % in Douro and 13 % in Alentejo), carbon assimilation rates (PN; 72 % in Douro and 25 % in Alentejo), and the soluble sugar content of grapevine leaves, while decreasing the accumulation of plant growth regulators (ABA, IAA, and SA) during the ripening stage. The results show an up-regulation of ABA biosynthesis-related genes (VvNCED) in TF treated vines from the Douro vineyard mainly in 2017, suggesting an increased stress response under severe summer conditions. Additionally, kaolin triggered the expression of ABA-responsive genes (VvHVA22a and VvSnRK2.6) mainly in TF, indicating different varietal responses to kaolin application under fluctuating periods of summer stress.