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Publications

Publications by CAP

2020

iLoF: An intelligent Lab on Fiber Approach for Human Cancer Single-Cell Type Identification

Authors
Paiva, JS; Jorge, PAS; Ribeiro, RSR; Balmaña, M; Campos, D; Mereiter, S; Jin, C; Karlsson, NG; Sampaio, P; Reis, CA; Cunha, JPS;

Publication
Scientific reports

Abstract
With the advent of personalized medicine, there is a movement to develop "smaller" and "smarter" microdevices that are able to distinguish similar cancer subtypes. Tumor cells display major differences when compared to their natural counterparts, due to alterations in fundamental cellular processes such as glycosylation. Glycans are involved in tumor cell biology and they have been considered to be suitable cancer biomarkers. Thus, more selective cancer screening assays can be developed through the detection of specific altered glycans on the surface of circulating cancer cells. Currently, this is only possible through time-consuming assays. In this work, we propose the "intelligent" Lab on Fiber (iLoF) device, that has a high-resolution, and which is a fast and portable method for tumor single-cell type identification and isolation. We apply an Artificial Intelligence approach to the back-scattered signal arising from a trapped cell by a micro-lensed optical fiber. As a proof of concept, we show that iLoF is able to discriminate two human cancer cell models sharing the same genetic background but displaying a different surface glycosylation profile with an accuracy above 90% and a speed rate of 2.3 seconds. We envision the incorporation of the iLoF in an easy-to-operate microchip for cancer identification, which would allow further biological characterization of the captured circulating live cells.

2020

Femtosecond laser direct written off-axis fiber Bragg gratings for sensing applications

Authors
Viveiros, D; Amorim, VA; Maia, JM; Silva, S; Frazão, O; Jorge, PAS; Fernandes, LA; Marques, PVS;

Publication
Optics and Laser Technology

Abstract
First order off-axis fiber Bragg gratings (FBGs) were fabricated in a standard single mode fiber (SMF-28e) through femtosecond laser direct writing. A minimum offset distance between the grating and core center of 2.5 µm was found to create a multimode section, which supports two separate fiber modes (LP0,1 and LP1,1), each split into two degenerate polarization modes. The resulting structure breaks the cylindrical symmetry of the fiber, introducing birefringence (˜10-4) resulting in a polarization dependent Bragg wavelength for each mode. Based on the modal and birefringence behavior, three off-axis FBGs were fabricated with 3.0, 4.5 and 6.0 µm offsets from the core center, and then characterized in strain, temperature, and curvature. The tested off-axis FBGs exhibited a similar strain sensitivity of ~1.14 pm/µ? and a temperature sensitivity of ~12 pm/C. The curvature and orientation angle were simultaneously monitored by analyzing the intensity fluctuation and the wavelength shift of the LP1,1 Bragg resonance. A maximum curvature sensitivity of 0.53 dB/m-1 was obtained for the off-axis FBG with a 3.0 µm offset. © 2020 Elsevier Ltd

2020

Preface

Authors
Frazão, O; del Villar, I; Fabris, J; Gong, Y;

Publication
Optics & Laser Technology

Abstract

2020

Preface

Authors
Frazao, O; del Villar, I; Fabris, J; Gong, Y;

Publication
OPTICS AND LASER TECHNOLOGY

Abstract

2020

Tuning of Fiber Optic Surface Reflectivity through Graphene Oxide-Based Layer-by-Layer Film Coatings

Authors
Monteiro, CS; Raposo, M; Ribeiro, PA; Silva, SO; Frazao, O;

Publication
Photonics

Abstract
The use of graphene oxide-based coatings on optical fibers are investigated, aiming to tune the reflectivity of optical fiber surfaces for use in precision sensing devices. Graphene oxide (GO) layers are successfully deposited onto optical fiber ends, either in cleaved or hollow microspheres, by mounting combined bilayers of polyethylenimine (PEI) and GO layers using the Layer-by-Layer (LbL) technique. The reflectivity of optical fibers coated with graphene oxide layers is investigated for the telecom region allowing to both monitor layer growth kinetics and cavity characterization. Tunable reflective surfaces are successfully attained in both cleaved optical fibers and hollow microsphere fiber-based sensors by simply coating them with PEI/GO layers through the LbL film technique.

2020

Curvature detection in a medical needle using a Fabry-Perot cavity as an intensity sensor

Authors
Novais, S; Silva, SO; Frazao, O;

Publication
Measurement: Journal of the International Measurement Confederation

Abstract
The use of optical sensors inside the needle can improve targeting precision and can bring real-time information about the location of the needle tip if necessary, since a needle bends through insertion into the tissue. Therefore, the precise location of the needle tip is so important in percutaneous treatments. In the current experiment, a fiber sensor based on a Fabry-Perot (FP) cavity is described to measure the needle curvature. The sensor is fabricated by producing an air bubble between two sections of multimode fiber. The needle with the sensor therein was attached at one end and deformed by the application of movements. The sensor presents a sensitivity of -0.152 dB/m-1 to the curvature measurements, with a resolution of 0.089 m-1. The sensory structure revealed to be stable, obtaining a cross-sensitivity to be 0.03 m-1/°C. © 2019 Elsevier Ltd

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