Abstract
Methylation is considered one of the proteins' most important post-translational modifications (PTM). Plasticity and cellular dynamics are among the many traits that are regulated by methylation. Currently, methylation sites are identified using experimental approaches. However, these methods are time-consuming and expensive. With the use of computer modelling, methylation sites can be identified quickly and accurately, providing valuable information for further trial and investigation. In this study, we propose a new machine-learning model called MeSEP to predict methylation sites that incorporates both evolutionary and structural-based information. To build this model, we first extract evolutionary and structural features from the PSSM and SPD2 profiles, respectively. We then employ Extreme Gradient Boosting (XGBoost) as the classification model to predict methylation sites. To address the issue of imbalanced data and bias towards negative samples, we use the SMOTETomek-based hybrid sampling method. The MeSEP was validated on an independent test set (ITS) and 10-fold cross-validation (TCV) using lysine methylation sites. The method achieved: an accuracy of 82.9% in ITS and 84.6% in TCV; precision of 0.92 in ITS and 0.94 in TCV; area under the curve values of 0.90 in ITS and 0.92 in TCV; F1 score of 0.81 in ITS and 0.83 in TCV; and MCC of 0.67 in ITS and 0.70 in TCV. MeSEP significantly outperformed previous studies found in the literature. MeSEP as a standalone toolkit and all its source codes are publicly available at https://github.com/arafatro/MeSEP.

Abstract
The integration of electric vehicles (EVs) into the power grid poses significant challenges and opportunities for energy management systems. This is especially concerning for parking lots or private building condominiums in which refurbishing is not possible or is costly. This paper presents a real-time monitoring approach to EV charging dynamics with battery storage support over a 24 h period. By simulating EV demand, state of charge (SOC), and charging and discharging events, we provide insights into the operational strategies for energy storage systems to ensure maximum charging simultaneity factor through internal power enhancement. The study uses a time-series analysis of EV demand, contrasting it with the battery's SOC, to dynamically adjust charging and discharging actions within the constraints of the upstream infrastructure capacity. The model incorporates parameters such as maximum power capacity, energy storage capacity, and charging efficiencies, to reflect realistic conditions. Results indicate that real-time SOC monitoring, coupled with adaptive charging strategies, can mitigate peak demands and enhance the system's responsiveness to fluctuating loads. This paper emphasizes the critical role of real-time data analysis in the effective management of energy resources in existing parking lots and lays the groundwork for developing intelligent grid-supportive frameworks in the context of growing EV adoption.