Lio Gonçalves

Abstract
Artificial intelligence is emerging in the field of veterinary medical imaging. The development of this area in medicine has introduced new concepts and scientific terminologies that professionals must be able to have some understanding of, such as the following: machine learning, deep learning, convolutional neural networks, and transfer learning. This paper offers veterinary professionals an overview of artificial intelligence, machine learning, and deep learning focused on imaging diagnosis. A review is provided of the existing literature on artificial intelligence in veterinary imaging of small animals, together with a brief conclusion.Artificial intelligence and machine learning have been increasingly used in the medical imaging field in the past few years. The evaluation of medical images is very subjective and complex, and therefore the application of artificial intelligence and deep learning methods to automatize the analysis process would be very beneficial. A lot of researchers have been applying these methods to image analysis diagnosis, developing software capable of assisting veterinary doctors or radiologists in their daily practice. This article details the main methodologies used to develop software applications on machine learning and how veterinarians with an interest in this field can benefit from such methodologies. The main goal of this study is to offer veterinary professionals a simple guide to enable them to understand the basics of artificial intelligence and machine learning and the concepts such as deep learning, convolutional neural networks, transfer learning, and the performance evaluation method. The language is adapted for medical technicians, and the work already published in this field is reviewed for application in the imaging diagnosis of different animal body systems: musculoskeletal, thoracic, nervous, and abdominal.

Abstract
Simple Summary Canine hip dysplasia development results in femoral neck modeling and an increase in thickness. The main objective of this work was to describe a femoral neck thickness index to quantify femoral neck width and to study its association with the degree of canine hip dysplasia using the Federation Cynologique Internationale scoring scheme. A total of 53 dogs (106 hips) were randomly selected for this study. Two examiners performed femoral neck thickness index estimation to study intra- and inter-examiner reliability and agreement. Statistical analysis tests showed excellent agreement and reliability between the measurements of the two examiners and the examiners' sessions. All joints were scored in five categories by an experienced examiner according to the Federation Cynologique Internationale criteria, and the results from examiner 1 were compared between these categories. The comparison of mean femoral neck thickness index between hip dysplasia categories using the analysis of variance test showed significant differences between groups. These results show that femoral neck thickness index is a parameter capable of evaluating proximal femur bone modeling and that it has the potential to enrich conventional canine hip dysplasia scoring criteria if incorporated into a computer-aided diagnosis software. The alteration in the shape of the femoral neck is an important radiographic sign for scoring canine hip dysplasia (CHD). Previous studies have reported that the femoral neck thickness (FNT) is greater in dogs with hip joint dysplasia, becoming progressively thicker with disease severity. The main objective of this work was to describe a femoral neck thickness index (FNTi) to quantify FNT and to study its association with the degree of CHD using the Federation Cynologique Internationale (FCI) scheme. A total of 53 dogs (106 hips) were randomly selected for this study. Two examiners performed FNTi estimation to study intra- and inter-examiner reliability and agreement. The paired t-test, the Bland-Altman plots, and the intraclass correlation coefficient showed excellent agreement and reliability between the measurements of the two examiners and the examiners' sessions. All joints were scored in five categories by an experienced examiner according to FCI criteria. The results from examiner 1 were compared between FCI categories. Hips that were assigned an FCI grade of A (n = 19), B (n = 23), C (n = 24), D (n = 24), and E (n = 16) had a mean & PLUSMN; standard deviation FNTi of 0.809 & PLUSMN; 0.024, 0.835 & PLUSMN; 0.044, 0.868 & PLUSMN; 0.022, 0.903 & PLUSMN; 0.033, and 0.923 & PLUSMN; 0.068, respectively (ANOVA, p < 0.05). Therefore, these results show that FNTi is a parameter capable of evaluating proximal femur bone modeling and that it has the potential to enrich conventional CHD scoring criteria if incorporated into a computer-aided diagnosis capable of detecting CHD.

Abstract
Adequate radiographic positioning on the X-ray table is paramount for canine hip dysplasia (HD) screening. The aims of this study were to evaluate femoral parallelism on normal ventrodorsal hip extended (VDHE) view and the effect of femoral angulation (FA) on Norberg Angle (NA) and Hip Congruency Index (HCI). The femoral parallelism was evaluated comparing the alignment of the long femoral axis with the long body axis in normal VDHE views and the effect of FA on NA and HCI on repeated VDHE views with different levels of FA. The femoral long axis in normal VDHE views showed a ranged of FA from -4.85 degrees to 5.85 degrees, mean +/- standard deviation (SD) of -0.06 +/- 2.41 degrees, 95% CI [-4.88, 4.76 degrees]. In the paired views, the mean +/- SD femur adduction of 3.69 +/- 1.96 degrees led to a statistically significant decrease NA, and HCI, and femur abduction of 2.89 +/- 2.12 led to a statistically significant increase in NA and HCI (p < 0.05). The FA differences were also significantly correlated with both NA differences (r = 0.83) and HCI differences (r = 0.44) (p < 0.001). This work describes a methodology that allows evaluation of femoral parallelism in VDHE views and the results suggest that femur abduction yielded more desirable NA and HCI values and adduction impaired NA and HCI values. The positive linear association of FA with NA and HCI allows the use of regression equations to create corrections, to reduce the influence of poor femoral parallelism in the HD scoring.

Abstract
Adequate radiographic positioning on the X-ray table is paramount for canine hip dysplasia (HD) screening. The aims of this study were to evaluate femoral parallelism on normal ventrodorsal hip extended (VDHE) view and the effect of femoral angulation (FA) on Norberg Angle (NA) and Hip Congruency Index (HCI). The femoral parallelism was evaluated comparing the alignment of the long femoral axis with the long body axis in normal VDHE views and the effect of FA on NA and HCI on repeated VDHE views with different levels of FA. The femoral long axis in normal VDHE views showed a ranged of FA from -4.85° to 5.85°, mean?±?standard deviation (SD) of -0.06?±?2.41°, 95% CI [-4.88, 4.76°]. In the paired views, the mean?±?SD femur adduction of 3.69?±?1.96° led to a statistically significant decrease NA, and HCI, and femur abduction of 2.89?±?2.12 led to a statistically significant increase in NA and HCI (p?&lt;?0.05). The FA differences were also significantly correlated with both NA differences (r?=?0.83) and HCI differences (r?=?0.44) (p?&lt;?0.001). This work describes a methodology that allows evaluation of femoral parallelism in VDHE views and the results suggest that femur abduction yielded more desirable NA and HCI values and adduction impaired NA and HCI values. The positive linear association of FA with NA and HCI allows the use of regression equations to create corrections, to reduce the influence of poor femoral parallelism in the HD scoring.

Abstract
This work aimed to define a humane endpoint scoring system able to objectively identify signs of animal suffering in a rat model of type 2 diabetes. Sprague-Dawley male rats were divided into control and induced group. The induced animals drink a 10% fructose solution for 14 days. Then, received an administration of streptozotocin (40 mg/kg). Animals’ body weight, water and food consumption were recorded weekly. To evaluate animal welfare, a score sheet with 14 parameters was employed. Blood glucose levels were measured at three time points. After seven weeks of initiating the protocol, the rats were euthanized. The induced animals showed weight loss, polyuria, polyphagia, and polydipsia. According to our humane endpoints table, changes in animal welfare became noticeable after the STZ administration. None of the animals hit the critical score limit (four). Data showed that the most effective parameters to assess welfare in this type 2 diabetes rat induction model were dehydration, grooming, posture, abdominal visualization, and stool appearance. The glycemia was significantly higher in the induced group when compared to the controls (p < 0.01). Induced animals’ murinometric and nutritional parameters were significantly lower than the controls (p < 0.01). Our findings suggest that in this rat model of type 2 diabetes with STZ-induced following fructose consumption, our list of humane endpoints is suitable for monitoring the animals’ welfare.