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Detalhes

Detalhes

  • Nome

    Rui Camacho
  • Cluster

    Informática
  • Cargo

    Investigador Sénior
  • Desde

    01 janeiro 2011
004
Publicações

2019

EvoPPI: A Web Application to Compare Protein-Protein Interactions (PPIs) from Different Databases and Species

Autores
Vazquez, N; Rocha, S; Lopez Fernandez, H; Torres, A; Camacho, R; Fdez Riverola, F; Vieira, J; Vieira, CP; Reboiro Jato, M;

Publicação
Practical Applications of Computational Biology and Bioinformatics, 12th International Conference, PACBB 2018, Toledo, Spain, 20-22 May, 2018.

Abstract
Biological processes are mediated by protein-protein interactions (PPI) that have been studied using different methodologies, and organized as centralized repositories - PPI databases. The data stored in the different PPI databases only overlaps partially. Moreover, some of the repositories are dedicated to a species or subset of species, not all have the same functionalities, or store data in the same format, making comparisons between different databases difficult to perform. Therefore, here we present EvoPPI (http://evoppi.i3s.up.pt), an open source web application tool that allows users to compare the protein interactions reported in two different interactomes. When interactomes belong to different species, a versatile BLAST search approach is used to identify orthologous/paralogous genes, which to our knowledge is a unique feature of EvoPPI. © Springer Nature Switzerland AG 2019.

2019

EvoPPI 1.0: a Web Platform for Within- and Between-Species Multiple Interactome Comparisons and Application to Nine PolyQ Proteins Determining Neurodegenerative Diseases

Autores
Vazquez, N; Rocha, S; Lopez Fernandez, H; Torres, A; Camacho, R; Fdez Riverola, F; Vieira, J; Vieira, CP; Reboiro Jato, M;

Publicação
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES

Abstract
Protein-protein interaction (PPI) data is essential to elucidate the complex molecular relationships in living systems, and thus understand the biological functions at cellular and systems levels. The complete map of PPIs that can occur in a living organism is called the interactome. For animals, PPI data is stored in multiple databases (e.g., BioGRID, CCSB, DroID, FlyBase, HIPPIE, HitPredict, HomoMINT, INstruct, Interactome3D, mentha, MINT, and PINA2) with different formats. This makes PPI comparisons difficult to perform, especially between species, since orthologous proteins may have different names. Moreover, there is only a partial overlap between databases, even when considering a single species. The EvoPPI (http://evoppi.i3s.up.pt) web application presented in this paper allows comparison of data from the different databases at the species level, or between species using a BLAST approach. We show its usefulness by performing a comparative study of the interactome of the nine polyglutamine (polyQ) disease proteins, namely androgen receptor (AR), atrophin-1 (ATN1), ataxin 1 (ATXN1), ataxin 2 (ATXN2), ataxin 3 (ATXN3), ataxin 7 (ATXN7), calcium voltage-gated channel subunit alpha1 A (CACNA1A), Huntingtin (HTT), and TATA-binding protein (TBP). Here we show that none of the human interactors of these proteins is common to all nine interactomes. Only 15 proteins are common to at least 4 of these polyQ disease proteins, and 40% of these are involved in ubiquitin protein ligase-binding function. The results obtained in this study suggest that polyQ disease proteins are involved in different functional networks. Comparisons with Mus musculus PPIs are also made for AR and TBP, using EvoPPI BLAST search approach (a unique feature of EvoPPI), with the goal of understanding why there is a significant excess of common interactors for these proteins in humans.

2019

Empowering Distributed Analysis Across Federated Cohort Data Repositories Adhering to FAIR Principles

Autores
Rocha, A; Ornelas, JP; Lopes, JC; Camacho, R;

Publicação
ERCIM News

Abstract

2019

EvoPPI: A Web Application to Compare Protein-Protein Interactions (PPIs) from Different Databases and Species

Autores
Vazquez, N; Rocha, S; Lopez Fernandez, H; Torres, A; Camacho, R; Fdez Riverola, F; Vieira, J; Vieira, CP; Reboiro Jato, M;

Publicação
PRACTICAL APPLICATIONS OF COMPUTATIONAL BIOLOGY AND BIOINFORMATICS

Abstract
Biological processes are mediated by protein-protein interactions (PPI) that have been studied using different methodologies, and organized as centralized repositories - PPI databases. The data stored in the different PPI databases only overlaps partially. Moreover, some of the repositories are dedicated to a species or subset of species, not all have the same functionalities, or store data in the same format, making comparisons between different databases difficult to perform. Therefore, here we present EvoPPI (http://evoppi.i3s.up.pt), an open source web application tool that allows users to compare the protein interactions reported in two different interactomes. When interactomes belong to different species, a versatile BLAST search approach is used to identify orthologous/paralogous genes, which to our knowledge is a unique feature of EvoPPI.

2018

Using multi-relational data mining to discriminate blended therapy efficiency on patients based on log data

Autores
Rocha, A; Camacho, R; Ruwaard, J; Riper, H;

Publicação
Internet Interventions

Abstract

Teses
supervisionadas

2019

sistema de apoio à escolha de algoritmos para problemas de optimização

Autor
Pedro Manuel Correia de Abreu

Instituição
UP-FEUP

2019

A Toolbox for Genomic Studies

Autor
Carlos Miguel da Silva Pereira

Instituição
UP-FEUP

2019

Framework for genomic based cancer studies using Machine Learning algorithms

Autor
João Alexandre Gonçalinho Loureiro

Instituição
UP-FEUP

2019

Deep Learning for identification and quantification of oncocytic cells in microscopic images

Autor
Luís Telmo Soares Costa

Instituição
UP-FEUP

2019

Hybrid coding taxonomy for clinical search harmonization in Safe Havens

Autor
Michael André Pinto Domingues

Instituição
UP-FEUP