Luís Carlos Coelho

Abstract
Relative humidity (RH) monitorization is of extreme importance on scientific and industrial applications, and optical fiber sensors (OFS) may provide adequate solutions. Typically, these kinds of sensors depend on the usage of humidity responsive polymers, thus creating the need for the characterization of the optical and expansion properties of these materials. Four different polymers, namely poly(vinyl alcohol), poly(ethylene glycol), Hydromed™ D4 and microbiology agar were characterized and tested using two types of optical sensors. First, optical fiber Fabry–Perot (FP) tips were made, which allow the dynamical measurement of the polymers’ response to RH variations, in particular of refractive index, film thickness, and critical deliquescence RH. Using both FP tips and Long-Period fiber gratings, the polymers were then tested as RH sensors, allowing a comparison between the different polymers and the different OFS. For the case of the FP sensors, the PEG tips displayed excellent sensitivity above 80%RH, outperforming the other polymers. In the case of LPFGs, the 10% (wt/wt) PVA one displayed excellent sensitivity in a larger working range (60 to 100%RH), showing a valid alternative to lower RH environment sensing.

Abstract
Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template-assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, which are known to deform upon analyte binding. This work presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date only SPR-LFDs functionalized with antibodies have been reported. Here, the innovative combination of an SPR-LFD platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. It is worth noting that the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding a measurement cell suitable for a rapid and reliable setup, and easy for the scaling up of the measurements. Moreover, the fabrication process to realize the SPR platform is minimal, requiring only a metal deposition step.

Abstract
Biogenic amines (BAs) are well-known biomolecules, mostly for their toxic and carcinogenic effects. Commonly, they are used as an indicator of quality preservation in food and beverages since their presence in higher concentrations is associated with poor quality. With respect to BA’s metabolic pathways, time plays a crucial factor in their formation. They are mainly formed by microbial decarboxylation of amino acids, which is closely related to food deterioration, therefore, making them unfit for human consumption. Pathogenic microorganisms grow in food without any noticeable change in odor, appearance, or taste, thus, they can reach toxic concentrations. The present review provides an overview of the most recent literature on BAs with special emphasis on food matrixes, including a description of the typical BA assay formats, along with its general structure, according to the biorecognition elements used (enzymes, nucleic acids, whole cells, and antibodies). The extensive and significant amount of research that has been done to the investigation of biorecognition elements, transducers, and their integration in biosensors, over the years has been reviewed.

Abstract
Optical fiber gratings have long shown their sensing capabilities. One of the main challenges, however, is the interrogation method applied, since typical systems tend to use broadband light sources with optical spectrum analyzers, laser scanning units or CCD (Charged Coupled Device) spectrometers. The following paper presents the development of an interrogation system, which explores the temperature response of a multimode laser diode, in order to interrogate long period fiber gratings. By performing a spectral sweep along one of its rejection bands, a discrete attenuation spectrum is created. Through a curve fitting technique, the original spectrum is restored. The built unit, while presenting a substantially reduced cost compared with typical interrogation systems, is capable of interrogating along a 10 nm window with measurement errors reaching minimum values as low as 0.4 nm, regarding the grating central wavelength, and 0.4 dB for its attenuation. Given its low cost and reduced dimensions, the developed system shows potential for slow-changing field applications.

Abstract
Surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) are among the most common and powerful label-free refractive index-based biosensing techniques available nowadays. Focusing on LSPR sensors, their performance is highly dependent on the size, shape, and nature of the nanomaterial employed. Indeed, the tailoring of those parameters allows the development of LSPR sensors with a tunable wavelength range between the ultra-violet (UV) and near infra-red (NIR). Furthermore, dealing with LSPR along optical fiber technology, with their low attenuation coefficients at NIR, allow for the possibility to create ultra-sensitive and long-range sensing networks to be deployed in a variety of both biological and chemical sensors. This work provides a detailed review of the key science underpinning such systems as well as recent progress in the development of several LSPR-based biosensors in the NIR wavelengths, including an overview of the LSPR phenomena along recent developments in the field of nanomaterials and nanostructure development towards NIR sensing. The review ends with a consideration of key advances in terms of nanostructure characteristics for LSPR sensing and prospects for future research and advances in this field. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.