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Publicações

2022

Analysis of the Relative Humidity Response of Hydrophilic Polymers for Optical Fiber Sensing

Autores
Dias, B; Carvalho, J; Mendes, JP; Almeida, JMMM; Coelho, LCC;

Publicação
POLYMERS

Abstract
Relative humidity (RH) monitorization is of extreme importance on scientific and industrial applications, and optical fiber sensors (OFS) may provide adequate solutions. Typically, these kinds of sensors depend on the usage of humidity responsive polymers, thus creating the need for the characterization of the optical and expansion properties of these materials. Four different polymers, namely poly(vinyl alcohol), poly(ethylene glycol), Hydromed™ D4 and microbiology agar were characterized and tested using two types of optical sensors. First, optical fiber Fabry–Perot (FP) tips were made, which allow the dynamical measurement of the polymers’ response to RH variations, in particular of refractive index, film thickness, and critical deliquescence RH. Using both FP tips and Long-Period fiber gratings, the polymers were then tested as RH sensors, allowing a comparison between the different polymers and the different OFS. For the case of the FP sensors, the PEG tips displayed excellent sensitivity above 80%RH, outperforming the other polymers. In the case of LPFGs, the 10% (wt/wt) PVA one displayed excellent sensitivity in a larger working range (60 to 100%RH), showing a valid alternative to lower RH environment sensing.

2022

A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins

Autores
Arcadio, F; Seggio, M; Del Prete, D; Buonanno, G; Mendes, J; Coelho, LCC; Jorge, PAS; Zeni, L; Bossi, AM; Cennamo, N;

Publicação
NANOMATERIALS

Abstract
Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template-assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, which are known to deform upon analyte binding. This work presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date only SPR-LFDs functionalized with antibodies have been reported. Here, the innovative combination of an SPR-LFD platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. It is worth noting that the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding a measurement cell suitable for a rapid and reliable setup, and easy for the scaling up of the measurements. Moreover, the fabrication process to realize the SPR platform is minimal, requiring only a metal deposition step.

2022

Simple Optical Fiber Interferometer for Dynamic Measurement of Refractive Index and Thickness of Polymer Films

Autores
Dias, B; Mendes, JPS; de Almeida, JMMM; Coelho, LCC;

Publicação
IEEE SENSORS JOURNAL

Abstract

2022

Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept

Autores
Mendes, JP; Coelho, LCC; Jorge, PAS; Pereira, CM;

Publicação
BIOSENSORS-BASEL

Abstract
A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer's high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm/RIU and a FOM of 16 RIU-1. The low-cost and easy to build device was tested in a working range from 1 to 100 nmol/L, revealing a limit of detection (LOD) and a sensitivity of 0.25 nmol/L (0.037 mu g/mL) and 0.057 nm.L/nmol, respectively. The sensor also successfully differentiates the target analyte from the other abundant elements that are present in the human blood plasma.

2022

Optical Biosensor for the Detection of Biogenic Amines

Autores
Vasconcelos, HCASG; de Almeida, JMMM; Mendes, JP; Dias, B; Jorge, PAD; Saraiva, CMT; Coelho, LCC;

Publicação
IEEE SENSORS JOURNAL

Abstract