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Publicações

Publicações por PHT

2011

Visible and Infrared Optical Probes for Hemodynamic Parameters Assessment

Autores
Pereira, T; Oliveira, T; Cabeleira, M; Almeida, V; Borges, E; Cardoso, J; Correia, C; Pereira, HC;

Publicação
2011 IEEE SENSORS

Abstract
Four optical probes were developed to measure the arterial distension waveform generated by the ventricular contraction and assess clinically relevant information. The pressure wave propagates through the arterial tree and can be measured in the peripheral arteries. The probes make use of two distinct photo-detectors: planar and avalanche photodiodes. Independently, two different light sources were tested: visible and infrared light. Performance of the probes was evaluated in a test setup that simulates the fatty deposits commonly seen in the obese, between skin and the artery. The probes show good overall performance in the test setup with less than 8% root mean square error (RMSE). However, the probes lit with IR sources show better results for the more extreme cases, with a better resolution in the waveform, higher definition of notable points and higher SNR when compared to the visible source signals. In vivo, the IR probes allow easier waveform detection, even more relevant with the increasing of the deposit structures.

2011

OPTICAL METHODS FOR LOCAL PULSE WAVE VELOCITY ASSESSMENT

Autores
Pereira, T; Cabeleira, M; Matos, P; Borges, E; Almeida, V; Cardoso, J; Correia, C; Pereira, HC;

Publicação
BIOSIGNALS 2011

Abstract
Pulse wave velocity (PWV) is a clinically interesting parameter associated to cardiac risk due to arterial stiffness, generally evaluated by the time that the pressure wave spends to travel between two arbitrary points. Optic sensors are an attractive instrumental solution in this kind of time assessment applications due to their truly non-contact operation capability, which ensures an interference free measurement. On the other hand, they can pose different challenges to the designer, mostly related to the features of the signals they produce and to the associated signal processing burden required to extract error free, reliable information. In this work we evaluate two prototype optical probes dedicated to pulse transit time (PTT) evaluation as well as three algorithms for its assessment. Although the tests were carried out at the test bench, where "well behaved" signals can be obtained, the transition to a probe for use in humans is also considered. Results demonstrated the possibility of measuring pulse transit times as short as 1 ms with less than 1% error.

2010

Dynamic Interrogation of Long Period Gratings with Modulated Fibre Bragg Gratings

Autores
Carvalho, JP; Coelho, L; Correia, L; Frazao, O; Jorge, PAS; Baptista, JM; Dias, I; Santos, JL; Weyl, J; Pontes, MJ; Martinez, MA; Barbero, AP; Ribeiro, RM; Abelem, AJG; Giraldi, MTR;

Publicação
FOURTH EUROPEAN WORKSHOP ON OPTICAL FIBRE SENSORS

Abstract
It is reported a LPG dynamic interrogation technique based on the modulation of fibre Bragg gratings located in the readout unit that permits to attenuate the effect of the 1/f noise of the electronics in the resolution of the LPG-based sensing head. The concept is tested to detect variations of the external refractive index and a resolution of 2.0x10 (4) NIR was achieved without system optimization. Additionally, the effect in the sensor resolution when introducing Erbium and Raman optical amplification is experimentally investigated.

2010

Industrialization of advanced optical technologies for environmental monitoring

Autores
Frazao, O; Pereira, DA; Santos, JL; Dias, I; Dias, JM; Vaz, N; Teixeira, M; Quintela, A; Ferreira, J; Ferreira, LA; Araujo, FM;

Publicação
CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY

Abstract
In this work, an innovative fully integrated monitoring infrastructure based on optical fibre sensors was developed and implemented. In the framework of the research project named PROTEU [Tecnologias Avan double dagger adas para a Monitoriza double dagger o de Sistemas Estuarinos e Costeiros (PDCTM/P/MAR/15275/1999)], an 11 km optical fibre cable with Bragg sensors each 500 m was installed from the lagoon mouth to Vouga river, along the bed of the Espinheiro channel, allowing the real-time measurement of water temperature at each sensor location. The results of this project are currently feeding several studies concerning Ria de Aveiro and the surrounding area and are crucial for a continuous assessment and management of the environmental conditions. Meanwhile, a fibre optic sensing system for simultaneous measurement of temperature and salinity based on fibre Bragg grating (FBG) technology was also developed. In the following sections, a complete description of the fabrication process, as well as theoretical and experimental results regarding this particular sensing system, are addressed. Earlier in situ local measurements, as well as the latest remote monitoring and data processing scheme, are described. The developed technology is now being exploited by FiberSensing, an INESC Porto spin-off company devoted to the development of optical fibre Bragg grating-based sensor systems for advanced monitoring applications. The main markets of the company are the ones of structural health monitoring in civil and geotechnical engineering, energy production and distribution, and environment.

2010

Optical clearing of muscle with propylene glycol

Autores
Oliveira, L; Lage, A; Clemente, MP; Tuchin, VV;

Publicação
SARATOV FALL MEETING 2009: INTERNATIONAL SCHOOL FOR JUNIOR SCIENTISTS AND STUDENTS ON OPTICS, LASER PHYSICS, AND BIOPHOTONICS

Abstract
Skeletal muscle presents an internal fibrous structure. The existence of muscle fibers surrounded by interstitial fluid originates an internal step refractive index profile that causes light scattering. One way to minimize this effect inside a muscle is to perform an optical clearing treatment, using an adequate solution that presents a refractive index higher than the interstitial fluid. We have studied muscle spectral transmittance during sample immersion in propylene glycol. With the collection of transmittance spectra registered during a period of 20 minutes of immersion we could represent spectral transmittance evolution for several wavelengths and verify that the tissue samples have become more translucent. The optical clearing effect created in the tissue samples was characterized by an increase of 45% above the natural transmittance and the variations observed in tissue mass, pH and global refractive index. We also identified the initial mechanisms of agent diffusion into the tissue and consequent tissue dehydration from the spectral transmittance evolution. The histological analysis of variations caused in the internal structure of the tissues permitted to better explain the optical clearing effect created. Considering a mathematical model developed in previous studies, we could estimate the amount of agent that was inserted into the tissue samples.

2010

Rat muscle opacity decrease due to the osmosis of a simple mixture

Autores
Oliveira, L; Lage, A; Pais Clemente, MP; Tuchin, VV;

Publicação
JOURNAL OF BIOMEDICAL OPTICS

Abstract
It is known that the fibrous structure of muscle causes light scattering. This phenomenon occurs due to the refractive index discontinuities located between muscle fibers and interstitial fluid. To study the possibility of reducing light scattering inside muscle, we consider its spectral transmittance evolution during an immersion treatment with an optical clearing solution containing ethanol, glycerol, and distilled water. Our methodology consists of registering spectral transmittance of muscle samples while immersed in that solution. With the spectral data collected, we represent the transmittance evolution for some wavelengths during the treatment applied. Additionally, we study the variations that the treatment has caused on the samples regarding tissue refractive index and mass. By analyzing microscopic photographs of tissue cross section, we can also verify changes in the internal arrangement of muscle fibers caused by the immersion treatment. Due to a mathematical model that we develop, we can explain the variations observed in the studied parameters and estimate the amount of optical clearing agent that has diffused into the tissue samples during the immersion treatment. At the end of the study, we observe and explain the improvement in tissue spectral transmittance, which is approximately 65% after 20 min. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3486539]

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