2025
Autores
Teixeira, J; Lopes, T; Capela, D; Monteiro, CS; Guimaraes, D; Lima, A; Jorge, PAS; Silva, NA;
Publicação
SCIENTIFIC REPORTS
Abstract
Spectral Imaging techniques such as Laser-induced Breakdown Spectroscopy (LIBS) and Raman Spectroscopy (RS) enable the localized acquisition of spectral data, providing insights into the presence, quantity, and spatial distribution of chemical elements or molecules within a sample. This significantly expands the accessible information compared to conventional imaging approaches such as machine vision. However, despite its potential, spectral imaging also faces specific challenges depending on the limitations of the spectroscopy technique used, such as signal saturation, matrix interferences, fluorescence, or background emission. To address these challenges, this work explores the potential of using techniques from conventional RGB imaging to enhance the dynamic range of spectral imaging. Drawing inspiration from multi-exposure fusion techniques, we propose an algorithm that calculates a global weight map using exposure and contrast metrics. This map is then used to merge datasets acquired with the same technique under distinct acquisition conditions. With case studies focused on LIBS and Raman Imaging, we demonstrate the potential of our approach to enhance the quality of spectral data, mitigating the impact of the aforementioned limitations. Results show a consistent improvement in overall contrast and peak signal-to-noise ratios of the merged images compared to single-condition images. Additionally, from the application perspective, we also discuss the impact of our approach on sample classification problems. The results indicate that LIBS-based classification of Li-bearing minerals (with Raman serving as the ground truth), is significantly improved when using merged images, reinforcing the advantages of the proposed solution for practical applications.
2024
Autores
Teixeira, J; Ribeiro, J; Silva, N; Jorge, P;
Publicação
2024 IEEE SENSORS APPLICATIONS SYMPOSIUM, SAS 2024
Abstract
This paper describes the development of an optical tweezers system that operates in fully automatic mode. It features image recognition for particle tracking, allowing for the optical trapping and analysis of identified targets. The system can perform analysis of forward scattered light and Raman spectroscopy of the trapped particles, facilitating the automated analysis of a large number of samples without manual intervention. By leveraging combined analytical methods and AI for robust classification, this system contributes to the advancement of automated diagnostic tools. Preliminary results demonstrate the system's effectiveness using different kinds of standard and biofunctionalized PMMA microparticles.
2025
Autores
Capela, D; Lopes, T; Dias, F; Ferreira, MFS; Teixeira, J; Lima, A; Jorge, PAS; Silva, NA; Guimaraes, D;
Publicação
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
Abstract
Mineral identification is a challenging task in geological sciences, which often implies multiple analyses of the physical and chemical properties of the samples for an accurate result. This task is particularly critical for the mining industry, where proper and fast mineral identification may translate into major efficiency and performance gains, such as in the case of the lithium mining industry. In this study, a mineral identification algorithm optimized for analyzing lithium-bearing samples using Laser-induced breakdown spectroscopy (LIBS) imaging, is put to the test with a set of representative samples. The algorithm incorporates advanced spectral processing techniques-baseline removal, Gaussian filtering, and data normalization-alongside unsupervised clustering to generate interpretable classification maps and auxiliary charts. These enhancements facilitate rapid and precise labelling of mineral compositions, significantly improving the interpretability and interactivity of the user interface. Extensive testing on diverse mineral samples with varying complexities confirmed the algorithm's robustness and broad applicability. Challenges related to sample granulometry and LIBS resolution were identified, suggesting future directions for optimizing system resolution to enhance classification accuracy in complex mineral matrices. The integration of this advanced algorithm with LIBS technology holds the potential to accelerate the mineral evaluation, paving the way for more efficient and sustainable mineral exploration.
2025
Autores
Lopes, T; Cavaco, R; Capela, D; Dias, F; Teixeira, J; Monteiro, CS; Lima, A; Guimaraes, D; Jorge, PAS; Silva, NA;
Publicação
TALANTA
Abstract
Combining data from different sensing modalities has been a promising research topic for building better and more reliable data-driven models. In particular, it is known that multimodal spectral imaging can improve the analytical capabilities of standalone spectroscopy techniques through fusion, hyphenation, or knowledge distillation techniques. In this manuscript, we focus on the latter, exploring how one can increase the performance of a Laser-induced Breakdown Spectroscopy system for mineral classification problems using additional spectral imaging techniques. Specifically, focusing on a scenario where Raman spectroscopy delivers accurate mineral classification performance, we show how to deploy a knowledge distillation pipeline where Raman spectroscopy may act as an autonomous supervisor for LIBS. For a case study concerning a challenging Li-bearing mineral identification of spodumene and petalite, our results demonstrate the advantages of this method in improving the performance of a single-technique system. LIBS trained with labels obtained by Raman presents an enhanced classification performance. Furthermore, leveraging the interpretability of the model deployed, the workflow opens opportunities for the deployment of assisted feature discovery pipelines, which may impact future academic and industrial applications.
2024
Autores
Cavaco, R; Lopes, T; Jorge, PAS; Silva, NA;
Publicação
UNCONVENTIONAL OPTICAL IMAGING IV
Abstract
Spectral imaging is a technique that captures spectral information from a scene and maps it onto a 2D image, featuring the potential to reveal hidden features and properties of objects that are invisible to the human eye, such as elemental and molecular compositions. Augmented reality (AR), on the other hand, is a technology that enhances the perception of reality by superimposing digital information on the physical world. While these technologies have different purposes, they can be considered one and the same in terms of providing an user-centric extension of reality. Spectral imaging provides the information that can reveal the underlying nature of objects, while AR provides the method of visualization that can display the information in an intuitive and interactive way. In this work, we present a novel Unity toolkit that combines spectral imaging and a HoloLens 2 AR device to create an interactive and immersive experience for the user. The toolkit enables the interactive visualization of various elemental maps of a 3D rock model in AR using a simple and intuitive interface. With this technique, the user can select a sample model and an elemental map from a preloaded asset library and then see the map projected onto the rock model in AR, using simple interactions such as zoom adjustment, rotation, and pan of the models to explore features and properties in detail. The toolkit offers several advantages, including better contextual interpretation of the spectral data by placing it in relation to the shape and texture of the rock, increased user engagement and curiosity through the creation of a realistic and immersive experience, and ease of decision-making through the provision of comparative tools. In short, by combining spectral imaging and AR, we present an innovative approach that can enrich the user experience and expand the user knowledge of the environment.
2024
Autores
Mendes, JP; dos Santos, PSS; Dias, B; Núñez Sánchez, S; Pastoriza Santos, I; Pérez Juste, J; Pereira, CM; Jorge, PAS; de Almeida, JMMM; Coelho, LCC;
Publicação
ADVANCED OPTICAL MATERIALS
Abstract
Surface plasmon resonance (SPR) conventionally occurs at the interface of a thin metallic film and an external dielectric medium in fiber optics through core-guided light. However, this work introduces theoretical and experimental evidence suggesting that the SPR in optical fibers can also be induced through light scattering from Au nanoparticles (NPs) on the thin metallic film, defined as nanoparticle-induced SPR (NPI-SPR). This method adheres to phase-matching conditions between SPR dispersion curves and the wave vectors of scattered light from Au NPs. Experimentally, these conditions are met on an etched optical fiber, enabling direct interaction between light and immobilized Au NPs. Compared to SPR, NPI-SPR exhibits stronger field intensity in the external region and wavelength tuning capabilities (750 to 1250 nm) by varying Au NP diameters (20 to 90 nm). NPI-SPR demonstrates refractive index sensitivities of 4000 to 4416 nm per refractive index unit, nearly double those of typical SPR using the same optical fiber configuration sans Au NPs. Additionally, NPI-SPR fiber configuration has demonstrated its applicability for developing biosensors, achieving a remarkable limit of detection of 0.004 nm for thrombin protein evaluation, a twenty-fold enhancement compared to typical SPR. These findings underscore the intrinsic advantages of NPI-SPR for sensing. Surface plasmon resonance (SPR) typically occurs at the interface of a thin metallic film and a dielectric medium in fiber optics. This work presents evidence of nanoparticle-induced SPR (NPI-SPR) in optical fibers through light scattering from Au nanoparticles on the thin metallic film. NPI-SPR offers stronger field intensity, wavelength tuning, and enhanced refractive index sensitivities, making it advantageous for biosensing applications. image
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