Abstract

Abstract
Project-based learning offers a student centered pedagogy and a dynamic approach which promotes a deeper knowledge through real-world challenges and problems solving. The U.Openlab concept is developed at the University of Porto (U.Porto) in the context of the teaching/learning process and is assumed to be an interaction facilitator platform between real-world needs and practical works topics. The aim is to provide innovative answers and the effective use of assessment results, the credits recognition for future professional or scientific careers and a sustainable process oriented for, among others, universities heritage services' needs. The pilot-project in course at the University of Porto, focusing on the U.Porto Digital Museum, includes a technological infrastructure with three main components: several collections, an information and services management platform, with its corresponding digital repository; the U.Porto community contributions management platform (U.Porto OpenLab); and an adaptive framework that will support various interface applications with non-specific audiences. The U.Porto OpenLab considers two main areas: the bidding management area/calls for contributions and a contributions personal management area. The professor, the student and the "client" are the actors in the process. As for main tasks we point out the reception, selection and distribution of real needs/problems. Having concluded the assessment process, results were selected and an online publication was submitted. The inherent credits are recorded in a contributors' digital portfolio management and in the professional social networks profiles. The methodology will be presented and discussed as well as the process along with the obstacles and other factors contributing to the success and future replication of this experience.

Abstract
Innovation has a cost. It is often the case that museums trying to innovate, simply cannot afford what it takes to build truly memorable exhibitions. Lack of human resources and high-tech equipment makes it hard to create quality contents that could be shown to the general public. Nevertheless, universities' museums are usually embedded in an environment that has the potential to provide all the tools and human resources required. The only thing needed is to establish a proper strategy and an interaction facilitator platform - U.OpenLab - which enables creating, building and sharing knowledge about the museums' collections and the academic population. This will make it easier to distribute the aforementioned knowledge to the general public, in a truly sustainable, systematic, integrated and articulated manner. In this paper we are going to present the University of Porto (U.Porto) OpenLab prototype that is being built as the stepping stone of this project, providing students with learning in a project environment.

Abstract
Bone repair is a specialized type of wound repair controlled by complex multi-factorial events. The nervous system is recognized as one of the key regulators of bone mass, thereby suggesting a role for neuronal pathways in bone homeostasis. However, in the context of bone injury and repair, little is known on the interplay between the nervous system and bone. Here, we addressed the neuropeptide Y (NPY) neuronal arm during the initial stages of bone repair encompassing the inflammatory response and ossification phases in femoral-defect mouse model. Spatial and temporal analysis of transcriptional and protein levels of NPY and its receptors, Y1R and Y2R, reported to be involved in bone homeostasis, was performed in bone, dorsal root ganglia (DRG) and hypothalamus after femoral injury. The results showed that NPY system activity is increased in a time- and space-dependent manner during bone repair. Y1R expression was trigged in both bone and DRG throughout the inflammatory phase, while a Y2R response was restricted to the hypothalamus and at a later stage, during the ossification step. Our results provide new insights into the involvement of NPY neuronal pathways in bone repair.

Abstract

Abstract
The hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling. Fg-3D led to early infiltration of granulation tissue (6 days post-implantation), followed by bone defect closure, including periosteum repair (8 weeks post-injury). In the acute inflammatory phase (6 days) local gene expression analysis revealed significant increases of pro-inflammatory cytokines IL-6 and IL-8, when compared with non-operated animals. This correlated with modified proportions of systemic immune cell populations, namely increased T cells and decreased B, NK and NIT lymphocytes and myeloid cell, including the Mac 1+ (CD18+/CD11b+) subpopulation. At 8 weeks, Fg-3D led to decreased plasma levels of IL-1 beta and increased TGF-beta 1. Thus, our data supports the hypothesis, establishing a link between bone repair induced by Fg-3D and the immune response. In this sense, Fg-3D scaffolds may be considered immunomodulatory biomaterials.