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Publicações

Publicações por João Pedro Mendes

2022

Simple Optical Fiber Interferometer for Dynamic Measurement of Refractive Index and Thickness of Polymer Films

Autores
Dias, B; Mendes, JPS; de Almeida, JMMM; Coelho, LCC;

Publicação
IEEE SENSORS JOURNAL

Abstract
Fiber optic-based refractometers is a thoroughly researched field, with many different configurations being used. However, most designs require external calibration using substances of known refractive index (RI) and their fabrication process might be impractical and time consuming, creating the need for a quick and accurate method of measuring RI of different substances. A simple method for simultaneous measurement in real-time of RI and thickness of polymer thin films is presented, allowing dynamic measurements in the presence of changing environmental parameters, such as temperature or humidity. This method, which does not require previous calibration, is based on an inline Fabry-Perot (FP) cavity, created by dipping the tip of a cleaved optical fiber (OF) in a polymer solution. The procedure consists of using the equations of the low finesse FP interferometers to directly extract information from the structure created, such as RI and cavity length, by working in the spectral window from 1500 to 1600nm. The method was validated by creating FP cavities with liquids of known RI, for which a typical precision of 3 x 10(-3) was achieved, along with errors lower than 0.6% and 1% for RI and cavity length determination, respectively, The procedure was then used to monitor three different curing processes, namely the temperature curing of Sylgard (TM) 184, the UV curing of Norland Optical Adhesives (TM) 65 and the mixing and curing of Ceys (TM) Araldite epoxy glue. Both RI and cavity length were compared to reference values, showing excellent agreement with the experimental results for a method that does not require external calibration.

2022

Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept

Autores
Mendes, JP; Coelho, LCC; Jorge, PAS; Pereira, CM;

Publicação
BIOSENSORS-BASEL

Abstract
A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer's high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm/RIU and a FOM of 16 RIU-1. The low-cost and easy to build device was tested in a working range from 1 to 100 nmol/L, revealing a limit of detection (LOD) and a sensitivity of 0.25 nmol/L (0.037 mu g/mL) and 0.057 nm.L/nmol, respectively. The sensor also successfully differentiates the target analyte from the other abundant elements that are present in the human blood plasma.

2022

Optical biosensor for the detection of low concentrations of hydrogen peroxide in milk samples

Autores
Vasconcelos, H; Matias, A; Mendes, J; Arahjo, J; Dias, B; Jorge, PAS; Saraivaa, C; Coelho, LCC; de Almeida, JMMM;

Publicação
OPTICAL SENSING AND DETECTION VII

Abstract
A strategy for the detection of H2O2 as a milk adulterant using a single shot membrane sensor, is presented. Direct quantitative evaluation of H2O2 in raw, skimmed, semi-skimmed and whole milk was carried out based on a chemiluminescence reaction with luminol. For H2O2 water solutions a linear response was attained from 0.0001% to 0.007 %w/w, with a limit of detection of 3x10(-5) %w/w. A coefficient of determination, R-2, greater than 0.97 was achieved, with a relative standard deviation (RSD) not exceeding 10%. In the analyzed milk samples, the lowest H2O2 concentration detected was 0.001% w/w for raw and for skim milk and 0.002%w/w for, semi-skimmed and whole milk. The presented method is original, sensitive, rapid, and cost-effective. Due to the achieved sensitivity the method has great potential to be used for H2O2 detection in diverse areas, such as environmental monitoring and food quality.

2022

Optical Biosensor for the Detection of Biogenic Amines

Autores
Vasconcelos, HCASG; de Almeida, JMMM; Mendes, JP; Dias, B; Jorge, PAD; Saraiva, CMT; Coelho, LCC;

Publicação
IEEE SENSORS JOURNAL

Abstract
Biogenic amines (BAs) are compounds found in a vast range of food products. In recent years, there has been a crescent awareness toward food safety, followed by an increase in food regulations. Long-period fiber gratings (LPFGs) coated with titanium dioxide (TiO2) were used to monitor the optical properties of a layer of poly(ethylene-co-vinyl acetate) (PEVA) doped with maleic anhydride (MA), which was polymerized on top of TiO2. This hydrophobic polymeric structure is permeable to BA, which causes a steady increase in its effective refractive index (RI) causing a wavelength shift in the coated LPFG attenuation band. LPFG wavelength shift was observed and measured for the monoamine tyramine (TYR), to the diamines, putrescine (PUT), cadaverine (CAD), histamine (HIS), and tryptamine (TRYP), and to the polyamines, spermidine (SPED), and spermine (SPEM). It was determined that, while PEVA-coated devices present a residual sensitivity to BA, the MA greatly increases it. In fact, for PEVA only coated LPFGs, the sensitivities of 1.45 +/- 0.11, 0.97 +/- 0.05, 0.46 +/- 0.08, and 0.94 +/- 0.09 nmM-1 for PUT, CAD, HIS, and TYR, respectively, were measured. However, for PEVA-doped MA-coated LPFGs, the sensitivities are 3.34 +/- 0.13, 3.06 +/- 0.11, 2.62 +/- 0.14, and 3.65 +/- 0.23 nmM-1 for PUT, CAD, HIS, and TYR, respectively. Thus, the RI of PEVA increases with BAs in- diffusion, and MA doping further enhances the PEVA sensitivity to BA. The proposed sensor is expected to play a part in the further development of a biosensor for the quantification of BA in real foodstuff, providing a methodology for quality control.

2022

Optical Fiber Sensor for the Detection of Decarboxylation Products of Amino Acids

Autores
Vasconcelos, H; De Almeida, JMMM; Mendes, J; Dias, B; Jorge, PAS; Saraiva, C; Coelho, LCC;

Publicação
Optics InfoBase Conference Papers

Abstract
Long period fiber gratings coated with TiO2 and poly(ethylene-co-vinyl acetate) (PEVA), a polymeric structure permeable biogenic amines found in foodstuff, were used to detect these compounds through the wavelength shift of its attenuation band. © 2022 The Author(s).

2022

Optical fiber sensors for monitoring cement paste carbonation

Autores
Da Silva, PM; Mendes, JP; Coelho, LCC; De Almeida, JMMM;

Publicação
Journal of Physics: Conference Series

Abstract
The use of concrete has been widespread in our society in housing and infrastructure, despite the environmental cost associated with its production. Its decay poses a social, economic, and environmental problem. Currently, the carbonation of cement paste is monitored through the measurement of its pH, with several optical fiber sensors (OFS) have been produced for this purpose. In the current work the focus is, also, on the carbonation monitoring of cement paste through an OFS, but not through pH measurements. Single fiber reflectance spectroscopy, previously employed to measure cement paste durability, is used to monitor the discoloration of cement paste caused by carbonation. As the carbonation front reaches the fiber tip embedded in the cement paste, the signal reflected onto the fiber increases. The accelerated carbonation of two limestone cement paste samples in an atmosphere of 100% CO2 was successfully monitored. The applicability of the sensor for operational use with ambient CO2 was confirmed through the measurement of carbonation at 3% CO2. The cross interference from water ingress and egress was also evaluated, and it didn't hinder the measurements of carbonation. Therefore, a novel OFS capable of measuring cement paste carbonation and durability, was achieved. © Published under licence by IOP Publishing Ltd.

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