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Publicações

Publicações por LIAAD

2014

18th International Database Engineering & Applications Symposium, IDEAS 2014, Porto, Portugal, July 7-9, 2014

Autores
Desai, BC; Almeida, AMd; Bernardino, J; Gomes, EF;

Publicação
IDEAS

Abstract

2014

Preface

Autores
Almeida, AM; Gomes, EF; Bernardino, J;

Publicação
ACM International Conference Proceeding Series

Abstract

2014

Putative cis-regulatory drivers in colorectal cancer

Autores
Ongen, H; Andersen, CL; Bramsen, JB; Oster, B; Rasmussen, MH; Ferreira, PG; Sandoval, J; Vidal, E; Whiffin, N; Planchon, A; Padioleau, I; Bielser, D; Romano, L; Tomlinson, I; Houlston, RS; Esteller, M; Orntoft, TF; Dermitzakis, ET;

Publicação
Nature

Abstract
The cis-regulatory effects responsible for cancer development have not been as extensively studied as the perturbations of the protein coding genome in tumorigenesis. To better characterize colorectal cancer (CRC) development we conducted an RNA-sequencing experiment of 103 matched tumour and normal colon mucosa samples from Danish CRC patients, 90 of which were germline-genotyped. By investigating allele-specific expression (ASE) we show that the germline genotypes remain important determinants of allelic gene expression in tumours. Using the changes in ASE in matched pairs of samples we discover 71 genes with excess of somatic cis-regulatory effects in CRC, suggesting a cancer driver role. We correlate genotypes and gene expression to identify expression quantitative trait loci (eQTLs) and find 1,693 and 948 eQTLs in normal samples and tumours, respectively. We estimate that 36% of the tumour eQTLs are exclusive to CRC and show that this specificity is partially driven by increased expression of specific transcription factors and changes in methylation patterns. We show that tumour-specific eQTLs are more enriched for low CRC genome-wide association study (GWAS) P values than shared eQTLs, which suggests that some of the GWAS variants are tumour specific regulatory variants. Importantly, tumour-specific eQTL genes also accumulate more somatic mutations when compared to the shared eQTL genes, raising the possibility that they constitute germline-derived cancer regulatory drivers. Collectively the integration of genome and the transcriptome reveals a substantial number of putative somatic and germline cis-regulatory cancer changes that may have a role in tumorigenesis.

2014

Tandem RNA chimeras contribute to transcriptome diversity in human population and are associated with intronic genetic variants

Autores
Greger, L; Su, J; Rung, J; Ferreira, PG; Lappalainen, T; Dermitzakis, ET; Brazma, A; Geuvadis consortium,;

Publicação
PLoS ONE

Abstract
Chimeric RNAs originating from two or more different genes are known to exist not only in cancer, but also in normal tissues, where they can play a role in human evolution. However, the exact mechanism of their formation is unknown. Here, we use RNA sequencing data from 462 healthy individuals representing 5 human populations to systematically identify and in depth characterize 81 RNA tandem chimeric transcripts, 13 of which are novel. We observe that 6 out of these 81 chimeras have been regarded as cancer-specific. Moreover, we show that a prevalence of long introns at the fusion breakpoint is associated with the chimeric transcripts formation. We also find that tandem RNA chimeras have lower abundances as compared to their partner genes. Finally, by combining our results with genomic data from the same individuals we uncover intronic genetic variants associated with the chimeric RNA formation. Taken together our findings provide an important insight into the chimeric transcripts formation and open new avenues of research into the role of intronic genetic variants in post-transcriptional processing events. © 2014 Greger et al.

2014

Identification of genetic variants associated with alternative splicing using sQTLseekeR

Autores
Monlong, J; Calvo, M; Ferreira, PG; Guigó, R;

Publicação
Nature Communications

Abstract
Identification of genetic variants affecting splicing in RNA sequencing population studies is still in its infancy. Splicing phenotype is more complex than gene expression and ought to be treated as a multivariate phenotype to be recapitulated completely. Here we represent the splicing pattern of a gene as the distribution of the relative abundances of a geneâ(tm) s alternative transcript isoforms. We develop a statistical framework that uses a distance-based approach to compute the variability of splicing ratios across observations, and a non-parametric analogue to multivariate analysis of variance. We implement this approach in the R package sQTLseekeR and use it to analyze RNA-Seq data from the Geuvadis project in 465 individuals. We identify hundreds of single nucleotide polymorphisms (SNPs) as splicing QTLs (sQTLs), including some falling in genome-wide association study SNPs. By developing the appropriate metrics, we show that sQTLseekeR compares favorably with existing methods that rely on univariate approaches, predicting variants that behave as expected from mutations affecting splicing. © 2014 Macmillan Publishers Limited.

2014

Transcriptome characterization by RNA sequencing identifies a major molecular and clinical subdivision in chronic lymphocytic leukemia

Autores
Ferreira, PG; Jares, P; Rico, D; Gómez López, G; Martínez Trillos, A; Villamor, N; Ecker, S; González Pérez, A; Knowles, DG; Monlong, J; Johnson, R; Quesada, V; Djebali, S; Papasaikas, P; López Guerra, M; Colomer, D; Royo, C; Cazorla, M; Pinyol, M; Clot, G; Aymerich, M; Rozman, M; Kulis, M; Tamborero, D; Gouin, A; Blanc, J; Gut, M; Gut, I; Puente, XS; Pisano, DG; Martin Subero, JI; López Bigas, N; López Guillermo, A; Valencia, A; López Otín, C; Campo, E; Guigó, R;

Publicação
Genome Research

Abstract
Chronic lymphocytic leukemia (CLL) has heterogeneous clinical and biological behavior. Whole-genome and -exome sequencing has contributed to the characterization of the mutational spectrum of the disease, but the underlying transcriptional profile is still poorly understood. We have performed deep RNA sequencing in different subpopulations of normal B-lymphocytes and CLL cells from a cohort of 98 patients, and characterized the CLL transcriptional landscape with unprecedented resolution. We detected thousands of transcriptional elements differentially expressed between the CLL and normal B cells, including protein-coding genes, noncoding RNAs, and pseudogenes. Transposable elements are globally derepressed in CLL cells. In addition, two thousand genes-most of which are not differentially expressed-exhibit CLL-specific splicing patterns. Genes involved in metabolic pathways showed higher expression in CLL, while genes related to spliceosome, proteasome, and ribosome were among the most down-regulated in CLL. Clustering of the CLL samples according to RNA-seq derived gene expression levels unveiled two robust molecular subgroups, C1 and C2. C1/C2 subgroups and the mutational status of the immunoglobulin heavy variable (IGHV ) region were the only independent variables in predicting time to treatment in a multivariate analysis with main clinico-biological features. This subdivision was validated in an independent cohort of patients monitored through DNA microarrays. Further analysis shows that B-cell receptor (BCR) activation in the microenvironment of the lymph node may be at the origin of the C1/C2 differences. © 2014 Hansen et al.

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