Abstract
HARMONI is the first light visible and near-IR integral field spectrograph for the ELT. It covers a large spectral range from 450nm to 2450nm with resolving powers from 3500 to 18000 and spatial sampling from 60mas to 4mas. It can operate in two Adaptive Optics (AO) modes - SCAO (including a High Contrast capability) and LTAO - or with NOAO. The project is preparing for Final Design Reviews. The SCAO system for HARMONI is based on a pyramid wavefront sensor (PWFS) operating in the visible (700 - 1000 nm). Previous implementations on very large telescopes have demonstrated the challenges associated with optimising PWFS performance on-sky, particularly when operated at visible wavelengths. ELT operation will pose further challenges for AO systems, particularly related to the segmentation of the telescope and the control of badly seen 'etal modes'. In this paper we investigate these challenges in the context of the HARMONI SCAO system. We present the results of end-to-end simulations of our baseline approach, using a coupled control basis to avoid the runaway development of petal modes in the control loop. The impact of key parameters are investigated and methods for optical gain compensation and optimisation of the control basis are presented. We discuss recent updates to the control algorithms and demonstrate the possibility of improving performance using a form of super resolution. Finally, we report on the expected performance across a range of conditions.

Abstract
The instruments developed for the upcoming Extremely Large Telescopes (ELTs) will need efficient adaptive optics (AO) systems to correct the effects of the atmospheric turbulence and allow imaging at the highest angular resolution. One of the most important requirements for ELT AO-assisted instruments will be to deliver diffraction-limited images in a significant part of the sky. For that, the instruments will be equipped with laser guide stars (LGSs) providing most of the information required by AO instruments. But even with LGSs, AO systems still require the use of natural guide stars (NGSs) to compensate for image motion (jitter) and some low order aberrations. These NGSs are eventually limiting the fraction of the sky that can be achieved by AO systems, the so-called sky coverage (SC). We first present the SC assessment methods used for high angular resolution monolithic optical and near-infrared integral field spectrograph (HARMONI) and multiconjugate adaptive optics relay/multi-AO imaging camera for deep observations (MAORY/MICADO), that are both instruments for the ELT of the European Southern Observatory (ESO). They are based on a semianalytical description of the main contributors in the AO error budget, allowing for a fast estimation of the residual jitter. As such, these methods are well suited for statistical estimation of the SC on multiple science fields and/or to efficiently explore the system parameter space. We then compute the SC of the two instruments in cosmological fields from the cosmic assembly near-IR deep extragalactic legacy survey catalog. The goal is to provide an insight on the possibilities given by two different types of tomographic AO systems, i.e., laser tomography AO with HARMONI and multiconjugate AO with MAORY, on the same telescope. In particular, we show that HARMONI and MAORY/MICADO are complementary, meaning that the overall SC of ESO's ELT is much improved for applications common to both systems. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License.

Abstract
Current collaborative augmented reality (AR) systems establish a common localization coordinate frame among users by exchanging and comparing maps comprised of feature points. However, relative positioning through map sharing struggles in dynamic or feature-sparse environments. It also requires that users exchange identical regions of the map, which may not be possible if they are separated by walls or facing different directions. In this paper, we present Cappella11Like its musical inspiration, Cappella utilizes collaboration among agents to forgo the need for instrumentation, an infrastructure-free 6-degrees-of-freedom (6DOF) positioning system for multi-user AR applications that uses motion estimates and range measurements between users to establish an accurate relative coordinate system. Cappella uses visual-inertial odometry (VIO) in conjunction with ultra-wideband (UWB) ranging radios to estimate the relative position of each device in an ad hoc manner. The system leverages a collaborative particle filtering formulation that operates on sporadic messages exchanged between nearby users. Unlike visual landmark sharing approaches, this allows for collaborative AR sessions even if users do not share the same field of view, or if the environment is too dynamic for feature matching to be reliable. We show that not only is it possible to perform collaborative positioning without infrastructure or global coordinates, but that our approach provides nearly the same level of accuracy as fixed infrastructure approaches for AR teaming applications. Cappella consists of an open source UWB firmware and reference mobile phone application that can display the location of team members in real time using mobile AR. We evaluate Cappella across mul-tiple buildings under a wide variety of conditions, including a contiguous 30,000 square foot region spanning multiple floors, and find that it achieves median geometric error in 3D of less than 1 meter.

Abstract
The evaluation of the diffusion properties of optical clearing agents in biological tissues, which are necessary to characterize the transparency mechanisms, has been traditionally made using ex vivo tissues. With the objective of performing such evaluation in vivo, this study was made to evaluate and compare those properties for propylene glycol in skeletal muscle, as obtained with the collimated transmittance and diffuse reflectance kinetics. The diffusion time and the diffusion coefficient of propylene glycol in the muscle that were calculated both from transmittance and reflectance kinetics presented a deviation of 0.8%, a result that opens the possibility to use such a method in vivo. © 2022 IEEE.

Abstract
The direct calculation of the absorption coefficient spectra of various tissues from spectral measurements allowed to retrieve the contents of melanin and lipofuscin. In the rabbit brain cortex, 1.8 times higher melanin content is explained by the neuron degeneration process. Similar melanin and lipofuscin contents were found in the rabbit pancreas as a result of the tissue aging process. The conversion of 83 % of the melanin in the human normal kidney into lipofuscin in the cancer kidney indicates that lipofuscin can be considered a kidney cancer marker in humans. © 2022 IEEE.

Abstract
The pancreas is a highly important organ, since it produces insulin and prevents the occurrence of diabetes. Although rare, pancreatic cancer is highly lethal, with a small life expectancy after being diagnosed. The pancreas is one of the organs less studied in the field of biophotonics. With the objective of acquiring information that can be used in the development of future applications to diagnose and treat pancreas diseases, the spectral optical properties of the rabbit pancreas were evaluated in a broad-spectral range, between 200 and 1000 nm. The method used to obtain such optical properties is simple, based almost on direct calculations from spectral measurements. The optical properties obtained show similar wavelength dependencies to the ones obtained for other tissues, but a further analysis on the spectral absorption coefficient showed that the pancreas tissues contain pigments, namely melanin, and lipofuscin. Using a simple calculation, it was possible to retrieve similar contents of these pigments from the absorption spectrum of the pancreas, which indicates that they accumulate in the same proportion as a result of the aging process. Such pigment accumulation was camouflaging the real contents of DNA, hemoglobin, and water, which were precisely evaluated after subtracting the pigment absorption.