2009
Autores
Frazao, O; Aref, SH; Baptista, JM; Santos, JL; Latifi, H; Farahi, F; Kobelke, J; Schuster, K;
Publicação
IEEE PHOTONICS TECHNOLOGY LETTERS
Abstract
A fiber-optic Fabry-Perot sensing structure based on the utilization of a suspended-core fiber is presented. The interferometric structure is formed when a small length of the suspended-core fiber is spliced to the end of a standard single-mode fiber. The interfering waves are generated by the refractive-index mismatches between the two fibers in the splice region and at the end of the suspended-core fiber. Thermal and strain responses of two different sensing heads associated with suspended-core fibers with three and four holes are characterized.
2009
Autores
Ana Perez Herrera, RA; Frazao, O; Santos, JL; Araujo, FM; Ferreira, LA; Baptista, JM; Lopez Amo, M;
Publicação
IEEE SENSORS JOURNAL
Abstract
We report on the use of erbium doped fiber ( EDF) amplification to enhance a frequency modulated continuous wave (FMCW) technique for referencing optical intensity sensors located between two Bragg grating structures. The experiment combines the concept of FMCW with the spectrally selective mirror properties of Bragg gratings to interrogate with referencing properties intensity based sensors. The interrogation system without amplification yields a sensor resolution of around 0.078 dB. When the EDF amplifier is introduced into the experimental set up, the sensor sensitivity does not change, but the signal-to-noise ratio is improved, resulting into an enhanced resolution of 0.025 dB. We also obtain a remote sensing operation at a location of 50 km, showing the feasibility of this configuration to be used as a remote sensing application.
2009
Autores
Frazao, O; Caldas, P; Santos, JL; Marques, PVS; Turck, C; Lougnot, DJ; Soppera, O;
Publicação
OPTICS LETTERS
Abstract
A micrometric Fabry-Perot refractometer based on an end-of-fiber polymer tip is proposed. The fiber tip, with a length of 36 mu m, was fabricated by self-guiding photopolymerization. The two-wave interferometric operation was achieved by combining the light waves generated at the interface between the single-mode fiber and the polymer tip, and at the fiber tip end (Fresnel reflection). The Fabry-Perot interferometer is coherence addressed and heterodyne interrogated, resulting into a liquid refractive index resolution of approximate to 7.5 x 10(-4). (C) 2009 Optical Society of America
2009
Autores
Santos, JL; Oramas, E; Pego, AP; Granja, PL; Tomas, H;
Publicação
JOURNAL OF CONTROLLED RELEASE
Abstract
This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a p-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application.
2009
Autores
Viegas, D; Goicoechea, J; Santos, JL; Araujo, FM; Ferreira, LA; Arregui, FJ; Matias, IR;
Publicação
SENSORS
Abstract
This work addresses a new configuration that improves the sensitivity of a humidity sensor based on a long-period fiber grating coated with a SiO(2)-nanospheres film. An intermediate higher refractive index overlay, deposited through Electrostatic Self-Assembly, is placed between the fiber cladding and the humidity sensitive film in order to increase the total effective refractive index of the coating. With this intermediate design, a three-fold improvement in the sensitivity was obtained. Wavelength shifts up to 15 nm against 5 nm were achieved in a humidity range from 20% to 80%.
2009
Autores
Aref, SH; Amezcua Correa, R; Carvalho, JP; Frazao, O; Caldas, P; Santos, JL; Araujo, FM; Latifi, H; Farahi, F; Ferreira, LA; Knight, JC;
Publicação
OPTICS EXPRESS
Abstract
In this work, sensitivity to strain and temperature of a sensor relying on modal interferometry in hollow-core photonic crystal fibers is studied. The sensing structure is simply a piece of hollow-core fiber connected in both ends to standard single mode fiber. An interference pattern that is associated to the interference of light that propagates in the hollow core fundamental mode with light that propagates in other modes is observed. The phase of this interference pattern changes with the measurand interaction, which is the basis for considering this structure for sensing. The phase recovery is performed using a white light interferometric technique. Resolutions of +/- 1.4 mu epsilon and +/- 0.2 degrees C were achieved for strain and temperature, respectively. It was also found that the fiber structure is not sensitive to curvature. (C) 2009 Optical Society of America
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