Abstract
Measurements made in feces and urine of Wistar rats exposed to lead acetate (n?=?20) in drinking water since the fetal period were compared with those obtained from a control group (n?=?20) in order to assess the age influence on Pb excretion. The measurements were made in different collections of rats aging between 1 and 11?months. To determine the Pb content of the samples, total reflection X-ray fluorescence (TXRF) and electrothermal atomic absorption spectrometry (ETAAS) were used for the urine samples and energy dispersive X-ray fluorescence (EDXRF) was used for the feces.

Abstract
The concentration of lead in liver and kidneys of Wistar rats, fed with lead since fetal period in relation to their age and to a control group, was determined. A group of rats was exposed to lead acetate (n = 30) in drinking water and the other group was exposed to normal water (n = 20). Samples were collected from rats aging between 1 and 11 months and were analyzed by Energy Dispersive X-ray Fluorescence (EDXRF) without any chemical preparation. The EDXRF results were assessed by the PIXE (Proton Induced X-ray Emission) technique. The formaldehyde used to preserve the samples was also analyzed by ETAAS (Electro-Thermal Atomic Absorption Spectrometry) in order to verify if there was any loss of lead from the samples to the formaldehyde. We found that the loss was not significant (<2%). Concerning the mean values of the lead concentration measured in the contaminated soft tissues, in liver they range from 6 to 22 mu g g(-1), and in kidneys from 44 to 79 mu g g(-1). The control rats show, in general, values below the EDXRF detection limit (2 mu g g(-1)). The ratio kidney/liver ranges from 2 to 10 and is strongly positively correlated with the age of the animals. A Spearman correlation matrix to investigate the correlation between elemental concentrations and the dependence of these concentrations with age showed that there is a strong positive correlation with age for lead in the liver but not in the kidney. The correlation matrix showed also that the concentration of lead in these two soft tissues is not correlated. The lead accumulation in liver is made by different plateaus that strongly decrease with age. It was verified the existence of two levels of accumulation in kidney, not very highlighted, which might be indicative of a maximum accumulation level for lead in kidney.

Abstract
The accumulation of lead in several bones of Wistar rats with time was determined and compared for the different types of bones. Two groups were studied: a control group (n = 20), not exposed to lead and a contaminated group (n = 30), exposed to lead from birth, first indirectly through mother's milk, and then directly through a diet containing lead acetate in drinking water (0.2%). Rats age ranged from 1 to 11 months, with approximately 1 month intervals and each of the collections had 3 contaminated rats and 2 control rats. Iliac, femur, tibia-fibula and skull have been analysed by Energy Dispersive X-ray Fluorescence Technique (EDXRF). Samples of formaldehyde used to preserve the bone tissues were also analysed by Electrothermal Atomic Absorption (ETAAS), showing that there was no significant loss of lead from the tissue to the preservative. The bones mean lead concentration of exposed rats range from 100 to 300 mu g g(-1) while control rats never exceeded 10 mu g g(-1). Mean bone lead concentrations were compared and the concentrations were higher in iliac, femur and tibia-fibula and after that skull. However, of all the concentrations in the different collections, only those in the skull were statistically significantly different (p < 0.05) from the other types of bones. Analysis of a radar chart also allowed us to say that these differences tend to diminish with age. The Spearman correlation test applied to mean lead concentrations showed strong and very strong positive correlations between all different types of bones. This test also showed that mean lead concentrations in bones are negatively correlated with the age of the animals. This correlation is strong in iliac and femur and very strong in tibia-fibula and skull. It was also shown that the decrease of lead accumulation with age is made by three plateaus of accumulation, which coincide, in all analysed bones, between 2nd-3rd and 9th-10th months.

Abstract

Abstract
A Fabry-Perot (FP) cavity of simple design and based on a pure silica diaphragm-free hollow tube is proposed. Its operation is based on a first reflection of light at the end of the single-mode fiber that illuminates the silica rod and in a second reflection that takes place at the end of the rod. The FP cavity is characterized for high temperature, pressure and refractive index sensing, showing useful characteristics for the measurement of these three parameters. The diaphragm-free configuration simplifies the measurement of the refractive index of fluids. (C) 2011 Optical Society of America

Abstract
The underground movement of water through soil and rock is an important phenomenon in Civil Engineering. Its study is made more appealing to students if small scale prototypes are used, where several layouts of soil and water in steady state/transient conditions can be studied in detail. A water tank prototype was built with a reflective optical fibre pressure sensor based on a GRIN lens and a mirror. The mirror is connected to an elastic membrane that is deformed when water pressure is applied and the lens is correctly aligned with the mirror and fixed. The distance between the mirror and the lens changes, so the reflective optical power changes as well and it is directly proportional to the water pressure inside the tank. The results obtained for water pressure up to 4 kPa for filling and emptying operations show that the sensor has a linear response for pressure changes between 1.7 kPa and 3.4 kPa with a slope of 181 mu W/kPa for filling and 191 mu W/kPa for emptying. It is also observed some hysteresis that may possibly be reduced by choosing another material for the membrane. Using this type of sensor head it is possible to monitor different pressure points in the small scale prototype using the standard OTDR (Optical Time Domain Reflectometer) equipment.