Abstract
In this manuscript we explore the feasibility of using LWUV-VIS-SWNIR (340 - 1100 nm) spectroscopy to classify Saccharomyces cerevisiae colony structures in YP agar and YPD agar, under different growth conditions, such as: i) no alcohol; ii) 1% (nu/nu) Ethanol; iii) 1% (nu/nu) 1-Propanol; iv) 1% (nu/nu) 1-butanol; v) 1 % (nu/nu) Isopropanol; vi) 1% (nu/nu) (+/-)-1-Phenylethanol; vii) 1% (nu/nu) Isoamyl alcohol; viii) 1% (nu/nu) tert-Amyl alcohol (2-Methyl-2-butanol); and ix) 1% (nu/nu) Amyl alcohol. Results show that LWUV-VISSWNIR spectroscopy has the potential for yeasts metabolic state identification once the spectral signatures of colonies differs from each others, being possible to acheive 100% of classification in UV-VIS and VIS-SWNIR. The UV-VIS region present high discriminant information (350-450 nm), and different responses to UV excitation were obtained. Therefore, high precision is obtained because UV-VIS and VIS-NIR exhibit different kinds of information. In the future, high precision analytical chemistry techniques such as mass spectroscopy and molecular biology transcriptomic studies should be performed in order to understand the detailed cell metabolism and genomic phenomena that characterize the yeast colony state.

Abstract
One of the most studied bioprocesses is fermentation by yeasts. Although this is true, there is still the lack of real-time instrumentation that is capable of providing detailed information on metabolic state of fermentations. In this research we explore the possibility of using UV-VIS-SWNIR spectroscopy as a high-output, non-destructive and multivariate methodology of monitoring beer fermentation. We herein report the implementation of the a fibber optics sensor and the capacity for detecting key parameters by partial least squares regression for biomass, extract, pH and total sugars. Results show that UV-VIS-SWNIR is a robust technique for monitoring beer fermentations, being able to provide detailed information spectroscopic fingerprinting of the process. Calibrations were possible to obtain for all the studied parameters with R2 of 0.85 to 0.94 in the UV-VIS region and 0.95 to 0.97 in the VIS-SWNIR region. This preliminary study allowed to conclude that further improvements in experimental methodology and signal processing may turn this technique into a valuable instrument for detailed metabolic studies in biotechnology.