Abstract
The influence of reduced feedback from the cutaneous receptors in the hindpaw in rat locomotion is still unclear. To evaluate this question., we conducted a detailed hindlimb kinematic analysis in animals, which suffered complete loss of thermal sensation. Two-dimensional hindlimb kinematics, temporal and spatial measurements. and walking track analysis were performed in rats before and during hypothermic anesthesia. The walking velocity. duration of the step cycle and stance phase, and stride length between the two testing conditions were statistically indistinguishable. Swing phase duration was significantly decreased during sensory loss. Analysis of angular motion revealed an increased hip and knee extension and an ankle joint with increased flexion during the step cycle under plantar anesthesia. Also after plantar cooling, the hip and knee angular velocity was significantly affected along the step cycle. The remarkably geometric similarity of the angle-angle plots obtained in our experiments reflected an interjoint coordination: however. the interpretation of the cyclogram perimeter revealed a larger excursion by the ankle and hip in their respective joint spaces in rats deprived of sensation. Examination of the horizontal position of the ankle with respect to the hip and the extension before toe-off revealed no major changes. whereas, there was a slight decrease in distance of the hip to the ground during sensory loss. Also, the walking tracks revealed a significant functional deficit following reduced cutaneous information of the plantar aspect of the hindpaw. We therefore conclude that sensory feedback from the hindpaw is important in the maintenance of normal rat locomotion.

Abstract
In experimental peripheral nerve studies, the rat sciatic nerve model is widely used to examine functional changes after different surgical repairs or pharmacological treatments, following nerve injury. The number and diversity of tests which have been used to assess functional recovery after experimental interventions often makes it difficult to recommend any particular indicator of nerve regeneration. Functional assessment after sciatic nerve lesion has long been focused on walking track analysis, therefore, this article describes in more detail the method to obtain and measure the walking tracks in order to calculate the sciatic functional index (SFI). However, it is important to note that the validity of the SFI has been questioned by several researchers. In addition, the present review includes other traditional tests described in the experimental peripheral nerve literature regarding the rate of return of motor function and sensation, such as the extensor postural thrust (EPT), nociceptive function, and the gastrocnemius-soleus weight parameters. In the last decade, several authors have designed a series of sensitive quantitative methods to assess the recovery of hind limb locomotor function using computerized rat gait analysis. This study aims to review kinematic measures that can be gathered with this technology, including calculation of sciatic functional index, gait-stance duration, ankle kinematics and toe out angle (TOA). A combination of tests, each examining particular components of recovered sensorimotor function is recommended for an overall assessment of rat sciatic nerve regeneration.

Abstract
The two-dimensional (2D) kinematic approach is by far the most popular technique in rat gait analysis. This is a simple inexpensive procedure, which requires only one camera to record the movement. However, maximal precision and accuracy of the kinematic values are expected when the experimental protocol includes a three-dimensional (3D) motion analysis methodology: Locomotor speed is a basic kinematic parameter that is often neglected in most studies of movement disorders and neurological diseases. Because locomotor speed c:an act as confounder for the interpretation of the obtained results we also focused our attention on the relation between speed and 3D hindlimb kinematics. Our experimental set-up consisted of a motion capture system with four CMOS cameras which allowed a non-invasive estimation of the instantaneous position of color markers in a 3D measurement volume. Data were recorded while rats walked at different treadmill speeds (30 vs 60 cm/s). For the first time we reported detailed kinematic data for the sagittal, coronal and transverse plane during treadmill locomotion in rats. Despite the overall time course patterns of the curves were identical, we found significant differences between values of joint angular motion at 30 and 60 cm/s at selected points of the step cycle. The adaptation to higher treadmill walking included greater joint angular excursions. The present report highlights the importance of walking speed when evaluating rat hindlimb kinematics during gait. Hopefully, this study will be useful in experimental data assessment when multiple gait abnormalities are expected to occur in all planes. of motion.

Abstract

Abstract
The Department of Biology of the University of Tras-os-Montes e Alto Douro analyses every year a large number of chestnut-tree leaves, in order to measure their biometric characteristics, namely the leaf area, dimensions of the enclosing rectangle, number of teeth and secondary veins. Because for a human operator this is a time consuming and error prone task, a computer vision system has been set up to improve the process. The task of measuring the leaf presents no major problems, while counting the number of teeth and secondary veins has proved to be complex at the resolutions used. This paper describes the state of the project, going into some detail on the algorithms. A complete system has been assembled, based on an PC connected to an imaging system. A windows-based application has been developed, which integrates the control of the operations to grab, store and analyse images of different varieties of chestnut-tree leaves in an organised way. Because the accuracy of the computer vision algorithms used is not sufficient for the system to be completely autonomous, a semi-automatic solution has been adopted. The operator validates or corrects the results of the automatic analysis. This solution leads to a significant improvement in the performance of the human operator, both in terms of speed and quality of the results.

Abstract
This study presents a method to measure the displacement fields on the surface of planar objects with sub-pixel resolution, by combining image correlation with a differential technique. First, a coarse approximation of the pixel level displacement is obtained by cross-correlation (CC). Two consecutive images, taken before and after the application of a given deformation, are recursively split in sub-images, and the CC coefficient is used as the similarity measure. Secondly, a fine approximation is performed to assess the sub-pixel displacements by means of an optical flow method based on a differential technique. To validate the effectiveness and robustness of the proposed method, several numerical tests were carried out on computer-generated images. Moreover, real images from a static test were also processed for estimating the displacement resolution. The results were compared with those obtained by a commercial digital image correlation code. Both methods showed similar and reliable results according to the proposed tests.