Abstract
Determining the PSF remains a key challenge for post adaptive-optics (AO) observations regarding the spatial, temporal and spectral variabilities of the AO PSF, as well as itx complex structure. This paper aims to provide a non-exhaustive but classified list of techniques and references that address this issue of PSF determination, with a particular scope on PSF reconstruction, or more generally pupil-plane-based approaches. We have compiled a large amount of references to synthesize the main messages and kept them at a top level. We also present applications of PSF reconstruction/models to post-processing, more especially PSF-fitting and deconvolution for which there is a fast progress in the community. © 2020 SPIE.

Abstract
On-sky testing of new instrumentation concepts is required before they can be incorporated within facility-class instrumentation with certainty that they will work as expected within a real telescope environment. Increasingly, many of these concepts are not designed to work in seeing-limited conditions and require an upstream adaptive optics system for testing. Access to on-sky AO systems to test such systems is currently limited to a few research groups and observatories worldwide, leaving many concepts unable to be tested. A pilot program funded through the H2020 OPTICON program offering up to 15 nights of on-sky time at the CANARY Adaptive Optics demonstrator is currently running but this ends in 2021. Pre-run and on-sky support is provided to visitor experiments by the CANARY team. We have supported 6 experiments over this period, and plan one more run in early 2021. We have recently been awarded for funding through the H2020 OPTICON-RADIO PILOT call to continue and extend this program up until 2024, offering access to CANARY at the 4.2m William Herschel Telescope and 3 additional instruments and telescopes suitable for instrumentation development. Time on these facilities will be open to researchers from across the European research community and time will be awarded by answering a call for proposals that will be assessed by an independent panel of instrumentation experts. Unlike standard observing proposals we plan to award time up to 2 years in advance to allow time for the visitor instrument to be delivered. We hope to announce the first call in mid-2021. Here we describe the facilities offered, the support available for on-sky testing and detail the eligibility and application process. © 2020 SPIE.

Abstract
Optical clearing (OC) opens new possibilities in enhancement of optical diagnostics of tumors. This chapter discusses different approaches for discrimination of healthy and malignant tissues using OC. The OC-assisted optical imaging techniques allow for acquiring high-resolution structural and functional images of neoplasms and their microvasculature. The study of the OC mechanisms in both normal and pathologically modified tissues provides differentiated results that allow for recognizing pathology. Both high water content and high cell density in tumors result in differences in diffusion rate of optical clearing agents (OCAs) in normal tissue and tumor. The monitoring of OCA diffusion in tissues with high temporal and depth resolution allows one to differentiate healthy from malignant tissues. Evaluation of protein dissociation during OC treatments may also help for discrimination between normal and pathological tissues.

Abstract
The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method, and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.

Abstract
The interest of using light in clinical practice is increasing strongly and many applications work at various wavelengths from the ultraviolet to the infrared. Due to this great range of applications, the determination of the optical properties of biological tissues in a wide spectral range becomes of interest. The liver is an important organ, since it has a major role in the human body and various pathologies are known to develop within it. For these reasons, this study concerns the estimation of the optical properties of human normal and pathological (metastatic carcinoma) liver tissues between 200 and 1000 nm. The obtained optical properties present the expected wavelength dependencies for both tissues - the refractive index, the absorption and the scattering coefficients decrease with the wavelength and the anisotropy and light penetration depth increase with the wavelength. Although similar behavior was observed for the various properties between the normal and pathological tissues, evidence of smaller blood content in the pathological tissues was found. A possible explanation is that the cancer cells destroy liver's vasculature and internal architecture, providing though a reduction in the blood content. For low wavelengths, it was observed a matching between the scattering and the reduced scattering coefficients, which implies a nearly zero anisotropy in that range. The scattering coefficient decreases from nearly 140 cm(-1) (at 200 nm) to 80 cm(-1) (at 1000 nm) for the normal liver and from nearly 140 cm(-1) (at 200 nm) to 95 cm(-1) (at 1000 nm) for the pathological tissue.

Abstract
The concept of 'tissue optical windows' and method of optical clearing (OC) based on controllable and reversible modification of tissue optical properties by their soaking with a biocompatible optical clearing agent (OCA) are prsented. Fundamentals and major mechanisms of OC allowing one to enhance optical imaging facilities and laser treatment efficiency of living tissues are described. Perspectives of immersion optical clearing/contrasting technique aiming to enhance optical imaging of living tissues by using different imaging modalities working in the ultra-broad wavelength range from deep UV to terahertz waves are discussed. It demonstrated that immersion OC method can be applied to evaluate the characteristic diffusion properties of water and OCA in various tissues and even discriminate between the mobile water content in normal and pathological tissues.