Abstract

Abstract
Spectral analysis of cultural heritage materials offers valuable insights into the restoration and preservation of historical artifacts, revealing details about the materials used and the manufacturing techniques employed. However, given their historical and artistic significance, the extraction of elemental information from these fragile samples poses a unique challenge, as these objects must be examined using minimally invasive methods to prevent irreversible damage. Laser-induced Breakdown Spectroscopy (LIBS) is one such technique, providing a rapid and detailed elemental characterization. Yet, extensive LIBS analysis can still compromise the integrity of these delicate objects. In this work, a novel approach that integrates spectral and RGB data clustering to significantly reduce the number of LIBS measurements required is introduced. By segmenting the material into visually and chemically distinct clusters, this method enables targeted LIBS analysis using only a few representative shots per cluster, thus preserving the integrity of cultural heritage artifacts while still delivering reliable compositional insights. (c) 2026 The Author(s). Published by Elsevier Masson SAS. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Abstract
The ability to assess molecular binding kinetics in real time is critical for advancing our understanding of molecular interactions in biochemical and biotechnological systems. This work presents a novel optical tweezer (OT)-based method to monitor molecular affinity in real time, focusing on the high-affinity streptavidin-biotin system as a model. Transparent poly(methyl methacrylate) (PMMA) microparticles functionalized with streptavidin were trapped before, during, and after binding with biotinylated bovine serum albumin (biotin-BSA), enabling the analysis of forward-scattered signals to detect nanoscale changes in particle size. By applying the Power Spectral Density method, the friction coefficient of individual particles was calculated, allowing for real-time tracking of binding dynamics and the estimation of the association rate constant (kon approximate to 106M-1s-1). These results are consistent with literature values and demonstrate the potential of this OT-based approach for non-invasive, label-free detection of molecular interactions. Compared to existing techniques, such as atomic force microscopy and cantilever-based sensors, this method offers significant advantages, including real-time monitoring, adaptability to different bioaffinity systems, and compatibility with miniaturized setups. This work establishes a foundation for using OT-based tools to monitor high-affinity molecular interactions in real time. While demonstrated here using biotinylated BSA as a model ligand, future studies will explore the method's applicability to smaller ligands and more subtle surface modifications.

Abstract
Over the past 18 months, we have performed hundreds of temperature characterizations of fiber Bragg gratings inscribed in different germanium-doped silica glass fibers. Under experimental conditions, the main conclusions are as follows: the temperature dependence of the temperature gauge factor or the normalized temperature sensitivity, K-T, was found to be quadratic in the -50-200 degrees C range, while it may be considered linear for the -20-100 degrees C range; K-T values at 20 degrees C vary from 5.176 x 10(-6) K-1, for a B/Ge co-doped fiber up to 6.724 x 10(-6) K-1, for a highly Ge-doped fiber; K-T does not depend on the hydrogen-loading process or the gratings coupling strength; K-T is essentially independent of wavelength in the 1500-1600 nm range, its value being accurately determined with a relative error similar to 0.2%; based on the accurate value of K-T = 6.165 x 10(-6) K-1, at 20 degrees C, obtained for gratings inscribed in the SMF-28 fiber, we calculated a value of 19.4 x 10(-6) K-1 for the thermo-optic coefficient of bulk germanium glass; and gratings produced by femtosecond-laser radiation and UV-laser radiation exhibit comparable values of K-T. The previous achievements allow, by having knowledge of K-T for a single grating, the accurate determination of the temperature dependence of the Bragg wavelength for any other grating inscribed in the same fiber; the presented methodology enables one to determine the unknown gratings' temperature sensitivity, typically with an error of 0.01 pm/degrees C, being, therefore, very useful in research labs and computer simulations. Thus, expressions for the temperature dependence of K-T for gratings inscribed in several fibers are given, as well as an expression for K-T as a function of the effective refractive index. We have also fully analyzed the potential sources of error in K-T determination.

Abstract
The decoupling of temperature and refractive index measurements was achieved by exploiting the properties of the asymmetric spectrum generated by Fano resonance, resulting from the interference between the Bragg reflection of the grating and the Fresnel reflection at the fiber tip. This spectral asymmetry enabled the implementation of a combined wavelength-based and intensity-based interrogation scheme. By separating the influence of each parameter in the spectral response, it was possible to measure the refractive index independently, without interference from temperature variations. A refractive index sensor with a minimum detectable change of delta = 1.2 & times; 10(-4) RIU was demonstrated. In addition to introducing a novel structure that leverages Fano resonance, the sensor was also applied as an evaporation rate sensor. The results demonstrate its potential for a wide range of applications, serving as a foundation for the development of future optical sensing technologies.

Abstract
This paper presents the conditions required for effective sensitivity amplification in the optical harmonic Vernier effect. Two distinct cases are analyzed: in the first, the sensor cavity is the shortest, while in the second, it is the longest. Based on the proposed theoretical model, supported by experimental results, it is concluded that, in the first case, the sensitivity associated with the spectral extremes increases with the order of the harmonic states. In contrast, in the second case, the sensitivity at the spectral extremes remains constant, regardless of the harmonic order. To evaluate the effectiveness of applying the optical Vernier effect and to differentiate between the two cases, a new formulation of the magnification factor (M-factor) is introduced. This leads to the definition of a novel figure of merit for the optical Vernier effect, denoted as (FoM(Vernier)). In Case 1, where harmonics are generated by increasing the reference cavity, the figure of merit assumes a value of (m + 1). In Case 2, where harmonics are generated by increasing the sensor cavity, the figure of merit remains constant at 1, regardless of the state order (whether fundamental or harmonic). This study also concludes that the observed increase in sensitivity is apparent rather than intrinsic, as the sensitivity curve produced by the optical Vernier effect mirrors that of a conventional interferometer.