2021
Autores
Rosolem, JB; Penze, RS; Floridia, C; Peres, R; Vasconcelos, D; Ramos Junior, MA;
Publicação
Sensors
Abstract
2021
Autores
Silva, AdA; Floridia, C; Rosolem, JB;
Publicação
Sensors
Abstract
2021
Autores
Rosolem, JB; Penze, RS; Floridia, C; Bassan, FR; Peres, R; Costa, EFd; de Araujo Silva, A; Coral, AD; Junior, JRN; Vasconcelos, D; Junior, MAR;
Publicação
IEEE Sensors Journal
Abstract
2021
Autores
Bassan, FR; Rosolem, JB; Floridia, C; Aires, BN; Peres, R; Aprea, JF; Nascimento, CAM; Fruett, F;
Publicação
Sensors
Abstract
2020
Autores
Paiva, JS; Jorge, PAS; Ribeiro, RSR; Balmana, M; Campos, D; Mereiter, S; Jin, CS; Karlsson, NG; Sampaio, P; Reis, CA; Cunha, JPS;
Publicação
SCIENTIFIC REPORTS
Abstract
With the advent of personalized medicine, there is a movement to develop "smaller" and "smarter" microdevices that are able to distinguish similar cancer subtypes. Tumor cells display major differences when compared to their natural counterparts, due to alterations in fundamental cellular processes such as glycosylation. Glycans are involved in tumor cell biology and they have been considered to be suitable cancer biomarkers. Thus, more selective cancer screening assays can be developed through the detection of specific altered glycans on the surface of circulating cancer cells. Currently, this is only possible through time-consuming assays. In this work, we propose the "intelligent" Lab on Fiber (iLoF) device, that has a high-resolution, and which is a fast and portable method for tumor single-cell type identification and isolation. We apply an Artificial Intelligence approach to the back-scattered signal arising from a trapped cell by a micro-lensed optical fiber. As a proof of concept, we show that iLoF is able to discriminate two human cancer cell models sharing the same genetic background but displaying a different surface glycosylation profile with an accuracy above 90% and a speed rate of 2.3 seconds. We envision the incorporation of the iLoF in an easy-to-operate microchip for cancer identification, which would allow further biological characterization of the captured circulating live cells.
2020
Autores
Viveiros, D; Amorim, VA; Maia, JM; Silva, S; Frazao, O; Jorge, PAS; Fernandes, LA; Marques, PVS;
Publicação
OPTICS AND LASER TECHNOLOGY
Abstract
First order off-axis fiber Bragg gratings (FBGs) were fabricated in a standard single mode fiber (SMF-28e) through femtosecond laser direct writing. A minimum offset distance between the grating and core center of 2.5 mu m was found to create a multimode section, which supports two separate fiber modes (LP0,1 and LP1,1), each split into two degenerate polarization modes. The resulting structure breaks the cylindrical symmetry of the fiber, introducing birefringence (approximate to 10(-4)) resulting in a polarization dependent Bragg wavelength for each mode. Based on the modal and birefringence behavior, three off-axis FBGs were fabricated with 3.0, 4.5 and 6.0 mu m offsets from the core center, and then characterized in strain, temperature, and curvature. The tested off-axis FBGs exhibited a similar strain sensitivity of similar to 1.14 pm/mu epsilon and a temperature sensitivity of similar to 12 pm/C. The curvature and orientation angle were simultaneously monitored by analyzing the intensity fluctuation and the wavelength shift of the LP1,1 Bragg resonance. A maximum curvature sensitivity of 0.53 dB/m(-1) was obtained for the off-axis FBG with a 3.0 mu m offset.
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